changing the LUT during image aquisition

[Ron Goldstein]

Hi,

I would like to set the "default" 8-bit LUT of ImageJ so that 255 (saturated) is red and 0 (black) is blue during acquisition with digital monochrome cameras. this is standard on confocal microscopes, and is a big aid in obtaining images with as wide a dynamic range as possible.
In the LUT directory, there is no "grays" LUT, and changing the LUT only affects images, not acquisition windows.
I tried the live histogram plugin and the plot line profile, and they only seem to work on images and not on live acquisition windows.
Is this something that can be changed in ImageJ, or is it something that the camera software needs to control?
If it can be done within ImageJ, how is it done?
Thanks very much,
-Ron
Dr. Ron Goldstein
Bar-Ilan University

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[Herbie]

Good day Ron,

the LUT-question for images opened in ImageJ is easy to answer:
You can build your own LUT in ImageJ. (Descriptions are in the User Guide.)

However, what you wish to see, is the application of a LUT during image
live acquisition and I fear that this is "something that the camera
software needs to control". I'm not sure if "Micro-Manager" provides
means for doing so and if "Micro-Manager" works with your hardware.

Best

Herbie

:::::::::::::::::::::::::::::::::::::::::::
Am 01.03.16 um 14:37 schrieb Ron Goldstein:

> Hi,
>
> I would like to set the "default" 8-bit LUT of ImageJ so that 255
> (saturated) is red and 0 (black) is blue during acquisition with
> digital monochrome cameras. this is standard on confocal microscopes,
> and is a big aid in obtaining images with as wide a dynamic range as
> possible. In the LUT directory, there is no "grays" LUT, and changing
> the LUT only affects images, not acquisition windows. I tried the
> live histogram plugin and the plot line profile, and they only seem
> to work on images and not on live acquisition windows. Is this
> something that can be changed in ImageJ, or is it something that the
> camera software needs to control? If it can be done within ImageJ,
> how is it done? Thanks very much, -Ron Dr. Ron Goldstein Bar-Ilan
> University
>
> -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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[Swayne, Theresa C.]

Hi Ron,

To add to Herbie’s good advice, the “HiLo" LUT is the one you want.

Image > Color > Display LUTs gives you a montage of all the LUTs on your system, and you can download more from the ImageJ site and others.

If you are using Micro-Manager to capture, there was a thread on their mailing list that might help:
http://micro-manager.3463995.n2.nabble.com/Custom-LUT-in-Live-Window-tp7581866p7581888.html

Hope this helps,
Theresa


On Mar 1, 2016, at 8:37 AM, Ron Goldstein <[hidden email]<mailto:[hidden email]>> wrote:

Hi,

I would like to set the "default" 8-bit LUT of ImageJ so that 255 (saturated) is red and 0 (black) is blue during acquisition with digital monochrome cameras. this is standard on confocal microscopes, and is a big aid in obtaining images with as wide a dynamic range as possible.
In the LUT directory, there is no "grays" LUT, and changing the LUT only affects images, not acquisition windows.
I tried the live histogram plugin and the plot line profile, and they only seem to work on images and not on live acquisition windows.
Is this something that can be changed in ImageJ, or is it something that the camera software needs to control?
If it can be done within ImageJ, how is it done?
Thanks very much,
-Ron
Dr. Ron Goldstein
Bar-Ilan University

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------------------------------------
Theresa Swayne, Ph.D.
Manager
Confocal and Specialized Microscopy Shared Resource<http://hiccc.columbia.edu/research/sharedresources/confocal>

Herbert Irving Comprehensive Cancer Center
Columbia University Medical Center
1130 St. Nicholas Ave., Room 222A
New York, NY 10032
Phone: [phone]
[hidden email]<mailto:[hidden email]>


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