colocalisation to measure contact surface.

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colocalisation to measure contact surface.

Amanda Sierra
Hi guys,

I'm a total begginer with ImageJ...

My question is: can I use the colocalization softtware to analyze the
contact surface between 2 different cell types? there is no actual
colocalisation there because the markers I am using are 100% specific for
each of the cell types. When I lower the threshold , the colocalization
mask reflects the contact surface, but I'm not sure if that's correct.
Is there any other (more appropriate) software to measure contact surface?
thanks a lot!!

amanda