corelation between pixel value to real scale

[sharmistha paul]

Hi everybody,

I've 8 bit SEM image. I calculated surface roughness using plugin. But it gives Rq value in pixel. How can I corelate this to real scale ( micrometer) ?
Please give some advice.

Cheers

Sharmistha


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[your name Charles Lessman]

With image open, select set scale from analyze menu and fill in drop
down. Then redo your calculations.

Charles A. Lessman, Ph.D.
Professor
Dept. of Biology
The University of Memphis
Memphis, TN 38152
[phone]
FAX [phone]
http://biology.memphis.edu/clessman.htm
http://umpeople.memphis.edu/clessman
 
-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
sharmistha paul
Sent: Wednesday, July 18, 2007 1:27 PM
To: [hidden email]
Subject: corelation between pixel value to real scale

Hi everybody,

I've 8 bit SEM image. I calculated surface roughness using plugin. But
it gives Rq value in pixel. How can I corelate this to real scale (
micrometer) ?
Please give some advice.

Cheers

Sharmistha


 
________________________________________________________________________
____________
Luggage? GPS? Comic books?
Check out fitting gifts for grads at Yahoo! Search
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[Gary Chinga]

I assume that if you have 8-bit SEM images then you are just calculating the greylevel variation of the images, not the real roughness values. For calculating the roughness (e.g. Rq) you need to reconstruct the surface, using e.g. SEM stereo imaging.

The Analyze/Set scale... will only calibrate the x,y dimensions, not the heights (z).

Gary.
http://www.gcsca.net 

>
>
> > From: Charles A Lessman (clessman) [[hidden email]]
> > Sent: 2007-07-18 20:53:40 CEST
> > To: [hidden email]
> > Subject: Re: corelation between pixel value to real scale
> >
> > With image open, select set scale from analyze menu and fill in drop
> > down. Then redo your calculations.
> >
> > Charles A. Lessman, Ph.D.
> > Professor
> > Dept. of Biology
> > The University of Memphis
> > Memphis, TN 38152
> > [phone]
> > FAX [phone]
> > http://biology.memphis.edu/clessman.htm
> > http://umpeople.memphis.edu/clessman
> >  
> > -----Original Message-----
> > From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
> > sharmistha paul
> > Sent: Wednesday, July 18, 2007 1:27 PM
> > To: [hidden email]
> > Subject: corelation between pixel value to real scale
> >
> > Hi everybody,
> >
> > I've 8 bit SEM image. I calculated surface roughness using plugin. But
> > it gives Rq value in pixel. How can I corelate this to real scale (
> > micrometer) ?
> > Please give some advice.
> >
> > Cheers
> >
> > Sharmistha
> >
> >
> >  
> > ________________________________________________________________________
> > ____________
> > Luggage? GPS? Comic books?
> > Check out fitting gifts for grads at Yahoo! Search
> > http://search.yahoo.com/search?fr=oni_on_mail&p=graduation+gifts&cs=bz
> >

[sharmistha paul]

Hi Prof. Lessman,

Thank you for your response.
Could you tell me please what is the pixel aspect ratio? Is it the ratio of two values shown by cursor when I move it on picture?
Regards,

Sharmistha


----- Original Message ----
From: Charles A Lessman (clessman) <[hidden email]>
To: [hidden email]
Sent: Wednesday, July 18, 2007 7:53:40 PM
Subject: Re: corelation between pixel value to real scale


With image open, select set scale from analyze menu and fill in drop
down. Then redo your calculations.

Charles A. Lessman, Ph.D.
Professor
Dept. of Biology
The University of Memphis
Memphis, TN 38152
[phone]
FAX [phone]
http://biology.memphis.edu/clessman.htm
http://umpeople.memphis.edu/clessman

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
sharmistha paul
Sent: Wednesday, July 18, 2007 1:27 PM
To: [hidden email]
Subject: corelation between pixel value to real scale

Hi everybody,

I've 8 bit SEM image. I calculated surface roughness using plugin. But
it gives Rq value in pixel. How can I corelate this to real scale (
micrometer) ?
Please give some advice.

Cheers

Sharmistha



________________________________________________________________________
____________
Luggage? GPS? Comic books?
Check out fitting gifts for grads at Yahoo! Search
http://search.yahoo.com/search?fr=oni_on_mail&p=graduation+gifts&cs=bz


       
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