Ceasar,
Well, you could just do it. Save the images in a folder and read them in using File/Import/Image Sequence...
Then do these steps to the resulting stack
1. Image/Type/8-bit.
2. Image/Color/Stack to RGB
3. Image/Adjust Brightness/Contrast... and decrease the Maximum from 255 to 108.
Now you have a very colorful image showing areas of black, red, green, blue, cyan, magenta, and white corresponding to the different combinations.
Steps 1 and 3 can be replaced with 1. Process/Binary/Make Binary, choosing Black Background. This a little more heavy-handed, and gives only 7 colors in the output, with no shading. It might be worth trying this approach for practice interpreting the colors. The version with shading looks clear too, but it almost seems like too much to ask to mentally operate in this mode. I have to admit that I don't know whether this is supposed to be possible, but it looks like it might be.
To keep track of what is what , note that the images in the stack are interpreted as Red, Green, and Blue in that order. You can influence the order by renaming them before importing.
Bob
On Apr 2, 2012, at 1:22 PM, Cesar Mendes wrote:
> Greetings,
>
> I am looking for a way to generate density maps from several (similar) neuronal images. Attached are images taken from three different animals of cell bodies plus dendritic fields. I would like to stack them all together in a single image so that a color code can tell me the degree of overlap. This way I can compare different genotypes.
> Any comment would help.
> Thanks in advance,
> Cesar
>
> <C.jpg><B.jpg><A.jpg>
Robert Dougherty, Ph.D.
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