densitometry and puncta count

[Achik Haim]

Hi there,

I'm interested in using ImageJ to analyze light microscopy (DAB) images for
densitometry and puncta count.
Is there a special plug-in for these functions or could I use the tools
available in my downloaded software?
Thanks!
Achik

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[Krs5]

Dear Achik,

Have a look at:
http://www.mecourse.com/landinig/software/cdeconv/cdeconv.html
http://jvsmicroscope.uta.fi/sites/default/files/software/immunomembrane-plugin/index.html
http://imagej.nih.gov/ij/plugins/ihc-toolbox/index.html
http://jvsmicroscope.uta.fi/sites/default/files/software/immunoratio-plugin/index.html

to get an idea what is out there.

Best wishes

Kees


Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif 


-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Achik Haim
Sent: 13 May 2014 18:29
To: [hidden email]
Subject: densitometry and puncta count

Hi there,

I'm interested in using ImageJ to analyze light microscopy (DAB) images for densitometry and puncta count.
Is there a special plug-in for these functions or could I use the tools available in my downloaded software?
Thanks!
Achik

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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[Gabriel Landini]

> I'm interested in using ImageJ to analyze light microscopy (DAB) images for
> densitometry and puncta count. Is there a special plug-in for these
> functions or could I use the tools available in my downloaded software?

Please be aware that unfortunately immunohistochemistry is not good for
densitometry.
This is explained in the colour deconvolution page posted by Kees,
http://www.mecourse.com/landinig/software/cdeconv/cdeconv.html
in the "Note" section.

Cheers

Gabriel

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