getting numbers out of gel lane plots

[Stuart Anderson-3]

Dear ImageJ experts -

Does anyone know how to export the numbers behind the plots ImageJ
produces from the PLOT LANES gel command?

Some context:

I'm trying to use ImageJ to process still images as part of a video
camera-based spectrometer.  The camera looks at the light dispersed
by a compact disc (i.e., the rainbow for white light).  From these
images I use the PLOT LANES feature in ImageJ to produce plots of
light intensity vs. position on the CD.

This works well for getting quick spectra, but I'd really like to get
a list of the numbers that ImageJ uses to make these plots for
subsequent processing - to produce an absorption spectrum, for
example, or for superposing / scaling several different spectra.

The TOOLS/SAVE X-Y COORDINATES function produces a list of the pixel
coordinates and their 8-bit intensities (pretty much 0 or 255 since
it's a line drawing).  I could process this list to get what I want,
but this seems crazy since ImageJ must have already calculated these
numbers to make the plots in the first place.

Is there a way?

Thanks!

Stu
--
*************************************************
Stuart M. Anderson
Associate Professor of Physics
Augsburg College
2211 Riverside Avenue
Minneapolis, MN  55454

Tel: [phone]
Internet: [hidden email]

****************************
"Saxophones are holey relics."
****************************

[Anatoly Shmygol]

Stu,
Try "plot profile" (Analyse->Plot Profile) on a rectangular or line selection of your image. This command gives you a graph with three buttons at the bottom: "List", "Save..." and "Copy"... These buttons will give access to the numbers you need. Rectangular selection will produce a better signal to noise ratio compared to the line selection but  you may need to rotate your image to make sure that your spectrum is oriented horizontally.
Regards,
Tony.
 
 A. Shmygol, MD, PhD.
Senior Lecturer (Reproductive Medicine)
Clinical Sciences Research Institute
University of Warwick Medical School
UHCW Campus
Clifford Bridge Road
Coventry   CV2 2DX
Tel.: [phone]
Fax: [phone]
e-mail: [hidden email]
>>> [hidden email] 03/28/06 09:28pm >>>
Dear ImageJ experts -

Does anyone know how to export the numbers behind the plots ImageJ
produces from the PLOT LANES gel command?

Some context:

I'm trying to use ImageJ to process still images as part of a video
camera-based spectrometer.  The camera looks at the light dispersed
by a compact disc (i.e., the rainbow for white light).  From these
images I use the PLOT LANES feature in ImageJ to produce plots of
light intensity vs. position on the CD.

This works well for getting quick spectra, but I'd really like to get
a list of the numbers that ImageJ uses to make these plots for
subsequent processing - to produce an absorption spectrum, for
example, or for superposing / scaling several different spectra.

The TOOLS/SAVE X-Y COORDINATES function produces a list of the pixel
coordinates and their 8-bit intensities (pretty much 0 or 255 since
it's a line drawing).  I could process this list to get what I want,
but this seems crazy since ImageJ must have already calculated these
numbers to make the plots in the first place.

Is there a way?

Thanks!

Stu
--
*************************************************
Stuart M. Anderson
Associate Professor of Physics
Augsburg College
2211 Riverside Avenue
Minneapolis, MN  55454

Tel:    [phone]
Internet:    [hidden email]

****************************
"Saxophones are holey relics."
****************************