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I am curious about the practical difference in feature/border enhancement
using the Filter/Unsharp Mask command compared to subtracting a separately
collected out-of-focus image. I haven't tried collecting these images to
compare the methods on my own - I was hoping that someone else already
has. If not, I'll try this out for some brightfield and fluorescent
images.
On a related note, Camp et al (Nature Medicine 8,1323-1328(2002) - detail in supplementary methods) discuss their use of RESA (rapid exponential subtraction algorithm) to
subtract their out-of-focus images. My understanding of how this works is
that an overall signal to noise ratio is determined for each single
channel image by dividing the value of the highest intensity pixel by that
of the lowest intesity pixel (or more likely the 1% and 99% pixels) and
this value is used to weight the amount of subtraction performed (is this
similar to th "Mask Weight" value?)
Is my understanding of this method correct? Would the addition of an
automatically calculated "Mask Weight" value for Unsharp Mask be useful?
Does such a plugin exist?
Thanks,
Jim Deeds
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