group z-projector

[Glen MacDonald-2]

Hello,

If a 4D dataset with 2 channels, z-series collected at time intervals, is opened as a Hyperstack, how can it be converted to a brightest point timelapse series?  the Grouped Z projector and the Grouped Stack projector merge both channels to create an 8-bit single channel movie unless I split the channels before using the grouped projectors.  

Any suggestions would be appreciated.  

Thanks,
Glen




Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
[phone]
[hidden email]

[Rasband, Wayne (NIH/NIMH) [E]]

On Jun 25, 2010, at 10:46 PM, Glen MacDonald wrote:

> Hello,
>
> If a 4D dataset with 2 channels, z-series collected at time intervals, is opened as a Hyperstack, how can it be converted to a brightest point timelapse series?  the Grouped Z projector and the Grouped Stack projector merge both channels to create an 8-bit single channel movie unless I split the channels before using the grouped projectors.  
>
> Any suggestions would be appreciated.  

The Image>Stacks>Z Project command works with hyperstacks. It can, for example, convert the c=2, z=5, t=51 Mitosis sample hyperstack to a c=2, z=51 brightest point t-series.

-wayne

[Glen MacDonald-2]

thanks Wayne,
That worked well!

Regards,
Glen
On Jun 26, 2010, at 5:07 AM, Rasband, Wayne (NIH/NIMH) [E] wrote:

> On Jun 25, 2010, at 10:46 PM, Glen MacDonald wrote:
>
>> Hello,
>>
>> If a 4D dataset with 2 channels, z-series collected at time intervals, is opened as a Hyperstack, how can it be converted to a brightest point timelapse series?  the Grouped Z projector and the Grouped Stack projector merge both channels to create an 8-bit single channel movie unless I split the channels before using the grouped projectors.  
>>
>> Any suggestions would be appreciated.  
>
> The Image>Stacks>Z Project command works with hyperstacks. It can, for example, convert the c=2, z=5, t=51 Mitosis sample hyperstack to a c=2, z=51 brightest point t-series.
>
> -wayne