how to generate random spots in 3D images by macro

[Wanzhu]

I have a 3D image and I demarcated a 3D ROI. I want to generate 200 spots (each spot's diameter is 4 um which represent cells) only placed in the 3D ROI. So I can further import this images to Imaris and calculate the distance between these simulated spots and actual blood vessels in ROI.  
I have no experience in Macro,  I really appreciate it if you can help me. Thank you.

[Thomas Boudier-4]

Hiu Wanzhu,

You can have a look to the 3D shape plugin :

http://imagejdocu.tudor.lu/doku.php?id=plugin:stacks:3d_tools:start

you have a macro to draw random ellipsoids at random positions, do not
hesitate to contact me if you need help to modify the macro to fit your
needs.

Best,

Thomas


On 03/08/2017 00:34, Wanzhu wrote:

> I have a 3D image and I demarcated a 3D ROI. I want to generate 200 spots
> (each spot's diameter is 4 um which represent cells) only placed in the 3D
> ROI. So I can further import this images to Imaris and calculate the
> distance between these simulated spots and actual blood vessels in ROI.
> I have no experience in Macro,  I really appreciate it if you can help me.
> Thank you.
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.com/how-to-generate-random-spots-in-3D-images-by-macro-tp5019155.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
>

--
  /***************************************************************/
       Thomas Boudier, Associate Professor, UPMC,
       Université Pierre et Marie Curie, Paris, France.
       BioInformatics Institute (BII)/IPAL, Singapore.
/**************************************************************/

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Wanzhu]

Thank you very much!

Thomas Boudier-4 wrote

Hiu Wanzhu,

You can have a look to the 3D shape plugin :

http://imagejdocu.tudor.lu/doku.php?id=plugin:stacks:3d_tools:start

you have a macro to draw random ellipsoids at random positions, do not
hesitate to contact me if you need help to modify the macro to fit your
needs.

Best,

Thomas


On 03/08/2017 00:34, Wanzhu wrote:
> I have a 3D image and I demarcated a 3D ROI. I want to generate 200 spots
> (each spot's diameter is 4 um which represent cells) only placed in the 3D
> ROI. So I can further import this images to Imaris and calculate the
> distance between these simulated spots and actual blood vessels in ROI.
> I have no experience in Macro,  I really appreciate it if you can help me.
> Thank you.
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.com/how-to-generate-random-spots-in-3D-images-by-macro-tp5019155.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
>

--
  /***************************************************************/
       Thomas Boudier, Associate Professor, UPMC,
       Université Pierre et Marie Curie, Paris, France.
       BioInformatics Institute (BII)/IPAL, Singapore.
/**************************************************************/

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html