image intensity correction

[yousef mazaheri]

Hi -

Is there a way to correct for signal non-uniformity across an image.

Thanks

Joe

[Elena Kardash]

Hi, Joe,
  try this: Process/Subtract Background

http://rsb.info.nih.gov/ij/docs/menus/process.html#background
the plugin is described here:
http://rsb.info.nih.gov/ij/plugins/background.html

 it works really nice

Elena


marefat wrote:

>Hi -
>
>Is there a way to correct for signal non-uniformity across an image.
>
>Thanks
>
>Joe
>  
>

[yousef mazaheri]

Hi Elena:

Thank you very much. It is excellent.

Thank you,
Yousef

On 2/10/06, Elena Kardash <[hidden email]> wrote:

> Hi, Joe,
>   try this: Process/Subtract Background
>
> http://rsb.info.nih.gov/ij/docs/menus/process.html#background
> the plugin is described here:
> http://rsb.info.nih.gov/ij/plugins/background.html
>
>  it works really nice
>
> Elena
>
>
> marefat wrote:
>
> >Hi -
> >
> >Is there a way to correct for signal non-uniformity across an image.
> >
> >Thanks
> >
> >Joe
> >
> >
>

[Guenter Giese]

Hi all:

a caveat: the plugin mentioned corrects for background (only). If your
brightness values (signal plus background) are lower in the edges, this may
be caused by the optical design of the instrument (illumination diminution
at the edges plus detection diminution, also known as vignetting in
photography). In this case you would have to apply some kind of
position-dependent correction implying multiplication or division, not
position-dependent offset correction.

Is there a plugin doing that?

Guenter

 

> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On
> Behalf Of marefat
> Sent: Samstag, 11. Februar 2006 00:16
> To: [hidden email]
> Subject: Re: image intensity correction
>
> Hi Elena:
>
> Thank you very much. It is excellent.
>
> Thank you,
> Yousef
>
> On 2/10/06, Elena Kardash <[hidden email]> wrote:
> > Hi, Joe,
> >   try this: Process/Subtract Background
> >
> > http://rsb.info.nih.gov/ij/docs/menus/process.html#background
> > the plugin is described here:
> > http://rsb.info.nih.gov/ij/plugins/background.html
> >
> >  it works really nice
> >
> > Elena
> >
> >
> > marefat wrote:
> >
> > >Hi -
> > >
> > >Is there a way to correct for signal non-uniformity across
> an image.
> > >
> > >Thanks
> > >
> > >Joe
> > >
> > >
> >
>

[Noel BONNET]

May be you could have a look at the plug-in
"A_posteriori_shading_correction" at:
http://www.univ-reims.fr/INSERM514/ImageJ

Hope it helps.

Noel


On Mon, 13 Feb 2006 09:43:58 +0100, Guenter Giese
<[hidden email]> wrote:

>Hi all:
>
>a caveat: the plugin mentioned corrects for background (only). If your
>brightness values (signal plus background) are lower in the edges, this may
>be caused by the optical design of the instrument (illumination diminution
>at the edges plus detection diminution, also known as vignetting in
>photography). In this case you would have to apply some kind of
>position-dependent correction implying multiplication or division, not
>position-dependent offset correction.
>
>Is there a plugin doing that?
>
>Guenter
>
>
>
>> -----Original Message-----
>> From: ImageJ Interest Group [mailto:[hidden email]] On
>> Behalf Of marefat
>> Sent: Samstag, 11. Februar 2006 00:16
>> To: [hidden email]
>> Subject: Re: image intensity correction
>>
>> Hi Elena:
>>
>> Thank you very much. It is excellent.
>>
>> Thank you,
>> Yousef
>>
>> On 2/10/06, Elena Kardash <[hidden email]> wrote:
>> > Hi, Joe,
>> >   try this: Process/Subtract Background
>> >
>> > http://rsb.info.nih.gov/ij/docs/menus/process.html#background
>> > the plugin is described here:
>> > http://rsb.info.nih.gov/ij/plugins/background.html
>> >
>> >  it works really nice
>> >
>> > Elena
>> >
>> >
>> > marefat wrote:
>> >
>> > >Hi -
>> > >
>> > >Is there a way to correct for signal non-uniformity across
>> an image.
>> > >
>> > >Thanks
>> > >
>> > >Joe
>> > >
>> > >
>> >
>>
>=========================================================================