Dear List,
I would like to measure increases in fluorescent intensity in
cross-sections of arteries, where a protein expressed only in the
endothelial cell layer is labelled. To the eye it appears as if the area
of the labelling is unchanged, it is just an increase in average
intensity (??more protein produced).
I find that thresholding for upper and lower ranges of significant
intensity within an ROI and measuring average intensity is not very
helpful because the abundance of low-mid range intensity pixels dilutes
the smaller contribution of the fewer bright pixels.
What I would like to do and my question is whether image J has an
automated plugin for this - to divide the significant intensity range
into 'bins', for example measure intensities (or histogram) of 50-70,
71-90, 91-110, etc. Perhaps plot the average of each bin and calculate
the slope. Then compare the treated and untreated groups. This way the
brightest (but fewest) pixels would have an equal contribution.
Any suggestions? Thank you.
Judy
Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 7Queen
30 Bond St.
Toronto, ON M5B 1W8, Canada
ph: 416-864-6060 x6337
pager: 416-685-9219
fax: 416-864-6043
[hidden email]
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