Hi, if you have already a binary image of the cell and of the gold
particles, the distance transform of the first (Process->Binary-
>Distance Map) redirected for measurement (Analyse->Set Measurement,
Analyse->Analyse Particle) with Integrated Density deviced by Area or
better Mean Gray Value delivers you per particle the shortest distance
to the cell border in pixels.
Regards
Karsten
Am 07.10.2008 um 09:40 schrieb Jan F. Richter:
> Dear all,
> I´m trying to measure the distance of immunogold-labelled proteins
> in cell using electron microscopy images. The experimental
> pertubation should affect the distance of the particles from the
> cell membrane. questions:
> 1.) is there something like a "leat-cost-routing " that
> automatically (or semi-automatically) finds the shortest path e.g.
> from the particle to the membrane ?
> 2.) could the membrane be "marked" as the reference to which the
> shortest path should be generated (the membrane would be represented
> by a line then) ?
>
> many thanks in advance !!
> best regards
> JFR
>
> --
> Dr. rer. medic. Jan F. Richter
> Institut f. Klinische Physiologie
> Charité / Campus Benjamin Franklin
> Hindenburgdamm 30
> 12200 Berlin
>
> Tel. 030-8445-4608
>
[hidden email]
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