Good day Susan,
I'd suggest that you perform the analyses/measurements sequentially,
i.e. for one square region after the other. For this purpose you move a
square-sized ROI of proper size over your image.
For this ROI-shifts you have to write an ImageJ-macro with loops that
gives you the ROI positions. A square-sized ROI is created by the command
makeRectangle(x, y, width, height);
Although it is possible to use scaled co-ordinates (um) I recommend to
use pixels.
Please consult the commented list of available macro functions at
<
https://imagej.nih.gov/ij/developer/macro/functions.html>
and the introduction to macro-coding at
<
https://imagej.nih.gov/ij/developer/macro/macros.html>
Good luck
Herbie
::::::::::::::::::::::::::::::::::::::::::::
Am 16.08.17 um 15:30 schrieb Travers, Susan:
> Hello,
>
> I would like to create a grid (for example, 500um wide and 300um high, with 50um squares) that I can then position at a desired location to be able to make measurement within the individual squares of the grid-I can envision how to do this using ROI's but not how to automate it...
> Does anyone have any suggestions?
>
> Thanks very much
>
> susan
>
> Susan Travers
> Professor, Biosciences
> Division of Biosciences, College of Dentistry
> The Ohio State University
> 305 West 12th Avenue
> Columbus, Ohio 43210
>
[hidden email]<mailto:
[hidden email]>
> (614)361-0800 (cell)
> (614)292-6366 (work)
>
>
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