merging chanels?

[Cammer, Michael]

Has there been a change in merging channels?

We used to adjust the contrast of 16 bit images to look the way we wanted them onthe screen and then merge them with Image > Color > Merge Channels... with composite off and Keep Source Images on.  If we split the resultant image later, we got 8 bit versions of the grayscale images we saw onthe monitor.

Today, using 1.45 with 32 bit Vista, the merged images are being contrast adjusted to look brighter, including features saturated, and splitting the channels shows that this was applied to the grayscale during the merge process.

For now we're converting to 8 bit before to get around this.

Thanks,

Michael

_________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]

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[Michael Schmid]

Hi Michael,

maybe you have changed the Edit>Options>Conversions 'Scale when
converting' settings?

Michael
_______________________________________________________________________

On Sat, March 19, 2011 19:11, Cammer, Michael wrote:

> Has there been a change in merging channels?
>
> We used to adjust the contrast of 16 bit images to look the way we wanted
> them onthe screen and then merge them with Image > Color > Merge
> Channels... with composite off and Keep Source Images on.  If we split the
> resultant image later, we got 8 bit versions of the grayscale images we
> saw onthe monitor.
>
> Today, using 1.45 with 32 bit Vista, the merged images are being contrast
> adjusted to look brighter, including features saturated, and splitting the
> channels shows that this was applied to the grayscale during the merge
> process.
>
> For now we're converting to 8 bit before to get around this.
>
> Thanks,
>
> Michael
>
> _________________________________________
> Michael Cammer, Assistant Research Scientist
> Skirball Institute of Biomolecular Medicine
> Lab: [phone]  Cell: [phone]
>
> ------------------------------------------------------------