metadata (again!?)

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metadata (again!?)

fabrice senger-2
Hi there,

this issue has been discussed several times , but...
I'm working on stk files...open them with LOCI...and everything works
fine...next I would like to extract creationTime data for each point in the
time serie in order to create a time slider...thus the info would be, e.g. :
t= 10 sec and not t 2/100.

Maybe this tool exist allready, maybe one will suggest to use the time
stamper...

Any suggestion is welcome, thank you,

Fabrice.
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Re: metadata (again!?)

vs11
Hi all ,
Would love to be able to pull time stamps too! I have 20gb experiments and it will make life easier. I am also using nd and stk files.
Thanks
Vinay
 

Vinay Singh
4315 FFSC, Duke university
Durham NC 27708
919-6134456

On Jul 16, 2010, at 4:14 AM, fabrice senger <[hidden email]> wrote:

> Hi there,
>
> this issue has been discussed several times , but...
> I'm working on stk files...open them with LOCI...and everything works
> fine...next I would like to extract creationTime data for each point in the
> time serie in order to create a time slider...thus the info would be, e.g. :
> t= 10 sec and not t 2/100.
>
> Maybe this tool exist allready, maybe one will suggest to use the time
> stamper...
>
> Any suggestion is welcome, thank you,
>
> Fabrice.
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Re: metadata (again!?)

Melissa Linkert-2
In reply to this post by fabrice senger-2
Hi Fabrice,

You could retrieve the timing data using macro code like this:

// -- start macro code ---

run("Bio-Formats Macro Extensions")

// choose a file
id = File.openDialog("Choose a file");

// display the images in the file
Ext.openImagePlus(id);

// read timing metadata from the file
Ext.setId(id);
// 'startDate' is in the ISO 8601 format
Ext.getImageCreationDate(startDate);
Ext.getImageCount(imageCount);
for (i=0; i<imageCount; i++) {
  // 'deltaT' is the time at which plane 'i' was acquired,
  // in seconds since 'startDate'
  Ext.getPlaneTimingDeltaT(deltaT, i);
}
Ext.close();

// -- end macro code --

This code should work for any format support by Bio-Formats, including
Metamorph STK.

Regards,
-Melissa

On Fri, Jul 16, 2010 at 10:14:16AM +0200, fabrice senger wrote:

> Hi there,
>
> this issue has been discussed several times , but...
> I'm working on stk files...open them with LOCI...and everything works
> fine...next I would like to extract creationTime data for each point in the
> time serie in order to create a time slider...thus the info would be, e.g. :
> t= 10 sec and not t 2/100.
>
> Maybe this tool exist allready, maybe one will suggest to use the time
> stamper...
>
> Any suggestion is welcome, thank you,
>
> Fabrice.
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quantification of cell area and orientation by ImageJ ?

yang-5
Hi,

Can someone give me a quick guide on how to use ImageJ to get the
quantification of cell area and orientation?   Thank you very much.

Sha Yang
Virginia Tech
United State
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Re: quantification of cell area and orientation by ImageJ ?

David Webster
Have you tried "Analyze/Analyze Particles..." and "Analyze/Set
Measurements..."

David Webster
On Mon, Jul 19, 2010 at 9:40 AM, yang <[hidden email]> wrote:

> Hi,
>
> Can someone give me a quick guide on how to use ImageJ to get the
> quantification of cell area and orientation?   Thank you very much.
>
> Sha Yang
> Virginia Tech
> United State