my problem with 'exclude edges' in analyze particles

[ctsiantar]

I'm creating a macro that will split up dots in a densely packed image and count them.
in order to maximise accuracy, I'm splitting the image up and optimising each region for the count.
the problem is, Analyze particles has an exclude edges function for all four edges, but not just two, thus I can either double count a load of dots, or miss out a load of dots completely

if there's a way to exclude only two edges, this would be ideal, although if anyone knows an alternative method that would also be great,

Thanks
Christian

[Bill Christens-Barry-2]

Christian,

Would it help to temporarily pad the image with an empty buffer strip along the two edges you wish not to be excluded from the touching prohibition?

Bill Christens-Barry

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Sidnei Paciornik]

Just use the Biovoxxel plugin (biovoxxel.de/macros.html) by Jan Brocher that
has a function specifically for that and a bunch of other very useful
functions.

Prof. Sidnei Paciornik
Grupo de Análise de Imagens e Microscopia Digital
DEMa <http://www.dema.puc-rio.br/> - Departamento de Engenharia de Materiais
PUC-Rio <http://www.puc-rio.br/>
Rua Marquês de São Vicente 225
Prédio Leme, Sala 501L
Gávea - Rio de Janeiro - RJ
22451-900 - Brasil
tel: [phone]





On Thu, Jul 18, 2013 at 4:37 PM, Bill Christens-Barry <[hidden email]>wrote:

> Christian,
>
> Would it help to temporarily pad the image with an empty buffer strip
> along the two edges you wish not to be excluded from the touching
> prohibition?
>
> Bill Christens-Barry
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Gabriel Landini]

The morphology collection has a "binary kill borders" plugin that erases
particles touching any border you wish.
http://www.dentistry.bham.ac.uk/landinig/software/software.html
Hope it is useful

Gabriel

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[ctsiantar]

Thanks for all the suggestions guys, worked around it in a totally different way in the end although did get it working with Christian's suggestion by alternating exclude edges between each tile, the final method just got higher accuracy.

Thanks again!