plugin nd stacks builder.......sorry I forgot to type in a subject for the previous query

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plugin nd stacks builder.......sorry I forgot to type in a subject for the previous query

nitya thiyagarajan
I am trying to open a metamorph-generated timelapse image stack (nd file)
from Image-J, through the "nd stack builder" plugin. This experiment
has about 10 stage positions, two wavelengths, 3 z planes for each
wavelength and about 90 time points. I am able to build the time series
stack after doing the desired z projection. However, I am not able to do it
for just one desired position and/or wavelength (even if I specify the
desired position and wavelength from the pulldown menu).  In other words,
the plug-in is always wanting all the sequential time point stacks for all
wavelengths and positions starting from position1, wavelength 1, timepoint 1
(it can do 1, 1-2, 1-3 etc, but not just for 2 or 3 only or the second
wavelength only). Does any one know if I can have this plugin to build only
the time series stack for the desired position and wavelength? (I have tried
changing the nd file code to make it read the experiment has having only one
position and wavelength, and it does show up as 1 position/1 wavelength when
I open it, but still is not able to build the stack). Any tips on this issue
will be greatly appreciated.



Thank you
Nitya Thiyagarajan
Graduate Research Assistant
UMKC- Dental School
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Re: plugin nd stacks builder.......sorry I forgot to type in a subject for the previous query

Fabrice Cordelières
Hi Nitya,

Here is the tip: whatever you choose in the pulldown menu, this choice won't
be taken in account unless you untick "All wavelength" and/or "All
timepoints" and/or "All positions". Depending on which boxes are unticked,
the process will be retricted to part of the dataset.

Fabrice

-----Message d'origine-----
De : ImageJ Interest Group [mailto:[hidden email]] De la part de nitya
thiyagarajan
Envoyé : vendredi 17 mars 2006 17:56
À : [hidden email]
Objet : plugin nd stacks builder.......sorry I forgot to type in a subject
for the previous query

I am trying to open a metamorph-generated timelapse image stack (nd file)
from Image-J, through the "nd stack builder" plugin. This experiment has
about 10 stage positions, two wavelengths, 3 z planes for each wavelength
and about 90 time points. I am able to build the time series stack after
doing the desired z projection. However, I am not able to do it for just one
desired position and/or wavelength (even if I specify the desired position
and wavelength from the pulldown menu).  In other words, the plug-in is
always wanting all the sequential time point stacks for all wavelengths and
positions starting from position1, wavelength 1, timepoint 1 (it can do 1,
1-2, 1-3 etc, but not just for 2 or 3 only or the second wavelength only).
Does any one know if I can have this plugin to build only the time series
stack for the desired position and wavelength? (I have tried changing the nd
file code to make it read the experiment has having only one position and
wavelength, and it does show up as 1 position/1 wavelength when I open it,
but still is not able to build the stack). Any tips on this issue will be
greatly appreciated.



Thank you
Nitya Thiyagarajan
Graduate Research Assistant
UMKC- Dental School