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Dear Kevin W Eliceiri and Wayne Rasband
Thank you for suggesting Bioformats Loci tools plugin.
I downloaded loci_tools.jar and placed in plugins folder and now image J is reading the ND2 file type but is displaying some gray background and no images.
To explain bit more I collected my images on Nikon 2000 with NIS elements software version 2.30 . I acquired a 4D image with option available in the software. green channel ,red channel phase contrast and merge of all the three together make 4 different images in the single ND2 file. Software alloted automatically 12 bit per each channel ( I do not know how to change this). I am able to open this ND2 file with NIS elements software but unable to open the ND2 files with Image J.
I would have mailed a sample image but mails with attachments are not delivered and are rejected.
sincerely
venu
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