pseudocolor mask for saturation

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pseudocolor mask for saturation

Glen MacDonald-2
Hello,
this may be a very basic RTFM question, but I've not found it.
How can saturated pixels be highlighted in a 16-bit gray scale image?  I'd like to pseudocolor them in a red mask to alert naive users.  Applying a threshold for 4095 (12-bit digitization)  is one workaround.  Sometimes a version of ImageJ/FIJI would pseudocolor saturated pixels in black and selecting 'Reset' in the B&C window would reset those to white.  

Thanks,
Glen
 


Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156
[hidden email]

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Re: pseudocolor mask for saturation

Stephan Saalfeld
Hi Glen,

have you tried the Lookup Table `HiLo'?

It still depends on the range that you select for visualization on
screen (min,max) but understanding this you get what you want.

Best,
Stephan



On Thu, 2012-06-14 at 10:59 -0700, Glen MacDonald wrote:

> Hello,
> this may be a very basic RTFM question, but I've not found it.
> How can saturated pixels be highlighted in a 16-bit gray scale image?  I'd like to pseudocolor them in a red mask to alert naive users.  Applying a threshold for 4095 (12-bit digitization)  is one workaround.  Sometimes a version of ImageJ/FIJI would pseudocolor saturated pixels in black and selecting 'Reset' in the B&C window would reset those to white.  
>
> Thanks,
> Glen
>  
>
>
> Glen MacDonald
> Core for Communication Research
> Virginia Merrill Bloedel Hearing Research Center
> Box 357923
> University of Washington
> Seattle, WA 98195-7923  USA
> (206) 616-4156
> [hidden email]
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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Re: pseudocolor mask for saturation

Glen MacDonald-2
In reply to this post by Glen MacDonald-2
Thank you Christine and Stephane,
that was what I was looking for.  Don't know how I missed it.

regards,
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156

On Thu, 14 Jun 2012, Christine Labno wrote:

> Hi Glen,
>
> Applying the HiLo look up table under Image -> Lookup Tables -> HiLo should do what you want.  It puts on red for saturated pixels and blue for 0 pixels and leaves all other values in grayscale.  I'm in ImageJ 1.46p for Windows XP.
>
> Best,
> Christine
>
> Christine Labno, Ph.D.
> Asst. Technical Director
> Light Microscopy Core
> University of Chicago
> Office of Shared Research Facilities
> KCBD 1250 900 E. 57th St.
> (773) 834-9040 (phone)
> Please consider the environment before printing this email.
>
>
> ---- Original message ----
>> Date: Thu, 14 Jun 2012 10:59:01 -0700
>> From: ImageJ Interest Group <[hidden email]> (on behalf of Glen MacDonald <[hidden email]>)
>> Subject: pseudocolor mask for saturation
>> To: <[hidden email]>
>>
>> Hello,
>> this may be a very basic RTFM question, but I've not found it.
>> How can saturated pixels be highlighted in a 16-bit gray scale image?  I'd like to pseudocolor them in a red mask to alert naive users.  Applying a threshold for 4095 (12-bit digitization)  is one workaround.  Sometimes a version of ImageJ/FIJI would pseudocolor saturated pixels in black and selecting 'Reset' in the B&C window would reset those to white.
>>
>> Thanks,
>> Glen
>>
>>
>>
>> Glen MacDonald
>> Core for Communication Research
>> Virginia Merrill Bloedel Hearing Research Center
>> Box 357923
>> University of Washington
>> Seattle, WA 98195-7923  USA
>> (206) 616-4156
>> [hidden email]
>>
>> --
>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html