pzfociez macro

[Krs5]

Dear list members,

I am looking for the macro pzfociez for gamma-H2AX foci analysis. It was written by Pawel Znojek and a search on the list and web links it to http://www.pzfociez.com/. However it looks like this website does not exist anymore. Does somebody know if there is a new download side for this macro or how I might be able to contact Pawel.

Many thanks

Kees


Dr Ir K.R. Straatman
Senior Experimental Officer
Centre for Core Biotechnology Services
University of Leicester
http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif


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[Ed Simmons]

On 16/04/14 08:43, Straatman, Kees (Dr.) wrote:
> Dear list members,
>
> I am looking for the macro pzfociez for gamma-H2AX foci analysis. It was written by Pawel Znojek and a search on the list and web links it to http://www.pzfociez.com/. However it looks like this website does not exist anymore. Does somebody know if there is a new download side for this macro or how I might be able to contact Pawel.
>
> Many thanks
>
> Kees
>
>
Hi Kees,

The wayback machine has it cached...
http://web.archive.org/web/20130621003236/http://pzfociez.com/

Hope that helps...
Ed

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[chin]

Hi Kees,

How about this link:
https://theses.ncl.ac.uk/dspace/bitstream/10443/1459/1/Znojek12.pdf

There seems the macro info that you want.

Best,
chin

[Krs5]

Hi Ed and list members,

As far as I can see the link only has the front page, any other links don't work and I don't see an option to download the macro.

Any change somebody has this macro on their system and could send it to me?

Best wishes

Kees

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Ed Simmons
Sent: 16 April 2014 08:54
To: [hidden email]
Subject: Re: pzfociez macro

On 16/04/14 08:43, Straatman, Kees (Dr.) wrote:
> Dear list members,
>
> I am looking for the macro pzfociez for gamma-H2AX foci analysis. It was written by Pawel Znojek and a search on the list and web links it to http://www.pzfociez.com/. However it looks like this website does not exist anymore. Does somebody know if there is a new download side for this macro or how I might be able to contact Pawel.
>
> Many thanks
>
> Kees
>
>
Hi Kees,

The wayback machine has it cached...
http://web.archive.org/web/20130621003236/http://pzfociez.com/

Hope that helps...
Ed

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[hidden email]
www.esimagingsolutions.com

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[Krs5]

Dear Chin,

Many thanks for the link. Seems to have all the info I need.

BW

Kees

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of chin
Sent: 16 April 2014 10:00
To: [hidden email]
Subject: Re: pzfociez macro

Hi Kees,

How about this link:
https://theses.ncl.ac.uk/dspace/bitstream/10443/1459/1/Znojek12.pdf

There seems the macro info that you want.

Best,
chin



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[pavanbn]

HI... could not find the plugin.. does anyone have a new link?? please share..

[Ion Andronache]

Please write to author
[hidden email].



________________________________________________________________
Best wishes,
Ion C. Andronache
Independent Researcher in fractal analysis and medical geography, Braila, Romania
PhD in geography (University of Bucharest, 2009)
https://www.researchgate.net/profile/Ion_Andronache3/
[hidden email]




22.05.2014, 14:12, "pavanbn" <[hidden email]>:
> HI... could not find the plugin.. does anyone have a new link?? please
> share..
>
> --
> View this message in context: http://imagej.1557.x6.nabble.com/pzfociez-macro-tp5007318p5007854.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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[Krs5]

Dear Pavan,

I will send you the macro off list as I received it from Peter van Loon after placing a request on this list.

Additional info can be found at: https://theses.ncl.ac.uk/dspace/bitstream/10443/1459/1/Znojek12.pdf (thanks to Chin for pointing me in that direction).

BW

Kees


Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of pavanbn
Sent: 22 May 2014 12:00
To: [hidden email]
Subject: Re: pzfociez macro

HI... could not find the plugin.. does anyone have a new link?? please share..



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[pavanbn]

Dear Ion C. Andronache,

The PDF file you sent was a bit confusing for a beginner, I was able to make the mask of my DAPI image, and then combine it with my H2AX staining - after then how do I proceed to count the foci per nuclei?

I clicked on the 'Fluorecence intensity measurement and counting' then on 'FociRB', it then open a dialog box to select the image, I select my H2AX staining picture - and it says 'File is not in a supported format, reader plugin not available, or it was not found'. My image was in .tiff format and was 8bit color image.

Also when I combined the mask of DAPI with H2AX, the outlines appeared around the nuclei (on my H2AX image). Then I chose the 'Find Maxima' option, all the foci inside the nucleus where marked and it counted the foci - but I want it for each nuclei.. Please help...

Just point out to a video if its available, or simply outline the steps I should follow.

Many thanks,
Pavan