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quantification of Polygon

nkxhm
6 posts
Hello there,
I am having a problem which may sounds silly but I can not find the solution. I do not know how to measure the polygon. I have a network with many polygons. Like in honeycomb with outline stroked. I want to find the distribution of area, circularity of the polygons. I can not achieve that with the particle analysis option since it is hallow.  Can some body help me out? I really appreciate that!

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Re: quantification of Polygon

G. Esteban Fernandez
65 posts
Do you have your objects defined correctly as black or white? If you get
unexpected results then invert your image (Image > Invert) and try again.

Or maybe your outlines are not wide enough; an open or close function might
help (Process > Binary > Open or Close).

-Esteban
On Mar 12, 2013 2:21 PM, "Huiming Xia" <[hidden email]> wrote:

> Hello there,
> I am having a problem which may sounds silly but I can not find the
> solution. I do not know how to measure the polygon. I have a network with
> many polygons. Like in honeycomb with outline stroked. I want to find the
> distribution of area, circularity of the polygons. I can not achieve that
> with the particle analysis option since it is hallow.  Can some body help
> me out? I really appreciate that!
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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Re: quantification of Polygon

nkxhm
6 posts
Hi Esteban,
Thank you very much for the response! I really appreciate your answer.
However, I can not find in my imageJ the invert option. I can not open or
close in the binary option either. Would you mind for further explaination?
Thank you so much!
Best regards,


On Tue, Mar 12, 2013 at 5:42 PM, G. Esteban Fernandez <
[hidden email]> wrote:

> Do you have your objects defined correctly as black or white? If you get
> unexpected results then invert your image (Image > Invert) and try again.
>
> Or maybe your outlines are not wide enough; an open or close function might
> help (Process > Binary > Open or Close).
>
> -Esteban
> On Mar 12, 2013 2:21 PM, "Huiming Xia" <[hidden email]> wrote:
>
> > Hello there,
> > I am having a problem which may sounds silly but I can not find the
> > solution. I do not know how to measure the polygon. I have a network with
> > many polygons. Like in honeycomb with outline stroked. I want to find the
> > distribution of area, circularity of the polygons. I can not achieve that
> > with the particle analysis option since it is hallow.  Can some body help
> > me out? I really appreciate that!
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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Re: quantification of Polygon

G. Esteban Fernandez
65 posts
Hi Huiming,

Thanks for providing an image, it's very helpful.  Your outlines are wide
enough, so don't mind what I said about binary open or close.  You just
need to convert your color image to a binary (black & white) image; try
this:

1.  Process > Binary > Make Binary
2.  Edit > Invert (you may not have to invert but do try it the first time)
3.  Analyze > Set Measurements, then select "Area" and "Shape descriptors"
and click OK.
4.  Analyze > Analyze Particles, here I'd use Show Outlines, Display
Results, Clear Results, and Summarize.

Let us know if this works or if you need further guidance.

-Esteban


On Tue, Mar 12, 2013 at 3:32 PM, Huiming Xia <[hidden email]> wrote:

> Hi Esteban,
> Thank you very much for the response! I really appreciate your answer.
> However, I can not find in my imageJ the invert option. I can not open or
> close in the binary option either. Would you mind for further explaination?
> Thank you so much!
> Best regards,
>
>
> On Tue, Mar 12, 2013 at 5:42 PM, G. Esteban Fernandez <
> [hidden email]> wrote:
>
> > Do you have your objects defined correctly as black or white? If you get
> > unexpected results then invert your image (Image > Invert) and try again.
> >
> > Or maybe your outlines are not wide enough; an open or close function
> might
> > help (Process > Binary > Open or Close).
> >
> > -Esteban
> > On Mar 12, 2013 2:21 PM, "Huiming Xia" <[hidden email]> wrote:
> >
> > > Hello there,
> > > I am having a problem which may sounds silly but I can not find the
> > > solution. I do not know how to measure the polygon. I have a network
> with
> > > many polygons. Like in honeycomb with outline stroked. I want to find
> the
> > > distribution of area, circularity of the polygons. I can not achieve
> that
> > > with the particle analysis option since it is hallow.  Can some body
> help
> > > me out? I really appreciate that!
> > >
> > > --
> > > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> > >
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html