quantification of mitochondria

[Anouk Waeytens]

Dear all,

I would like to quantify area, length, numbers etc of mitochondria in
mitochondrial networks (to look at fusion of mitochondria). I am looking for
the right plugins to convert a confocal 3D-image to a computerized
reconstruction and the right tools to analyze these recontstructions. Can
anyone help me?

thanks in advance!

Anouk

Anouk Waeytens

____________________________
Anouk Waeytens
Dept. of Pathology
De Pintelaan 185
B-9000 Gent
Belgium
tel [phone]
fax [phone]
e-mail: [hidden email] <mailto:[hidden email]>
____________________________

[dscho]

Hi,

On Tue, 28 Nov 2006, Anouk Waeytens wrote:

> I would like to quantify area, length, numbers etc of mitochondria in
> mitochondrial networks (to look at fusion of mitochondria). I am looking
> for the right plugins to convert a confocal 3D-image to a computerized
> reconstruction and the right tools to analyze these recontstructions.

As it happens, I had a closer look at mitochondria last week. I doubt that
you will find a plugin which just turns your confocal image (are you sure
you can see mitochondria on those?) into a reconstruction. This is _hard_!

Ciao,
Dscho

[Tony Collins-4]

VolumeJ will reconstruct your 3D data sets (not 4D) but if you're
wanting to analyse networks of mitochondria a functional assay may be
better. Using light microscopy of live cells you won't have the
resolution to see if mitochondria are connected or just touching. See:
T. J. Collins, M. J. Berridge, P. Lipp, and M. D. Bootman. Mitochondria
are morphologically and functionally heterogeneous within cells. EMBO J
21 (7):1616-1627, 2002.
T. J. Collins and M. D. Bootman. Mitochondria are morphologically
heterogeneous within cells. J Exp.Biol. 206 (Pt 12):1993-2000, 2003.

These papers have some mitochondrial 3d reconstructions using VolumeJ.
Contact me off list if you'd like further details.
Regards,
Tony

Tony J. Collins, Ph.D.
McMaster Biophotonics Facility
Dept. Biochemistry and Biomedical Sciences HSC 4H21A
McMaster University Hamilton, ON, [phone] ext. 28812
[hidden email]     www.macbiophotonics.ca

> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
> Anouk Waeytens
> Sent: Tuesday, November 28, 2006 6:05 AM
> To: [hidden email]
> Subject: quantification of mitochondria
>
> Dear all,
>
> I would like to quantify area, length, numbers etc of mitochondria in
> mitochondrial networks (to look at fusion of mitochondria). I am

looking
> for
> the right plugins to convert a confocal 3D-image to a computerized
> reconstruction and the right tools to analyze these recontstructions.
Can

> anyone help me?
>
> thanks in advance!
>
> Anouk
>
> Anouk Waeytens
>
> ____________________________
> Anouk Waeytens
> Dept. of Pathology
> De Pintelaan 185
> B-9000 Gent
> Belgium
> tel [phone]
> fax [phone]
> e-mail: [hidden email] <mailto:[hidden email]>
> ____________________________

... [show rest of quote]