ratiometric images

[shilo]

Dear list
I'm sending the massage again because I wasn't subscribed.

I would like to produce ratiometric images from two stacks. The two pictures come from confocal microscope and each one of them is from different excitation wavelength.
I was trying to make it with the calculator plus tool (divide), but I got image with 0,1,2 values and not continues values.
In the next step I would like to have colors that represent the ratio values and scaling bar

Thanks for your help
Shilo

[Michael Schmid]

Hi Shilo,

use Process>Image Calculator and check "Create New Window" and "32-
bit Result".
8-bit or 16-bit images can hold only integer numbers; "32-bit" is the  
floating point format.

For your other questions, see Image>Lookup Tables and  
Analyze>Tools>Calibration Bar (grayscale calibration) and  
Analyze>Tools>Scale Bar (spatial calibration).

Michael
________________________________________________________________

On 19 Nov 2008, at 18:35, shilo wrote:

> I would like to produce ratiometric images from two stacks. The two  
> pictures
> come from confocal microscope and each one of them is from different
> excitation wavelength.
> I was trying to make it with the calculator plus tool (divide), but  
> I got
> image with 0,1,2 values and not continues values.
> In the next step I would like to have colors that represent the  
> ratio values
> and scaling bar
>
> Thanks for your help
> Shilo

[Harry Parker]

Hello Shilo,

I'm guessing that if you first converted the images to floating point (menu: Image->Type->32 bit), you would get closer to the results you expect.

 
--
Harry Parker
Senior Imaging Systems Engineer
Digital Imaging Systems, Inc.



----- Original Message ----
From: shilo <[hidden email]>
To: [hidden email]
Sent: Wednesday, November 19, 2008 12:35:00 PM
Subject: ratiometric images

Dear list
I'm sending the massage again because I wasn't subscribed.

I would like to produce ratiometric images from two stacks. The two pictures
come from confocal microscope and each one of them is from different
excitation wavelength.
I was trying to make it with the calculator plus tool (divide), but I got
image with 0,1,2 values and not continues values.
In the next step I would like to have colors that represent the ratio values
and scaling bar

Thanks for your help
Shilo

--
View this message in context: http://n2.nabble.com/ratiometric-images-tp1519905p1519905.html
Sent from the ImageJ mailing list archive at Nabble.com.



     

[shilo]

Thank you all for your great help!!! it works.

Shilo

shilo wrote

Dear list
I'm sending the massage again because I wasn't subscribed.

I would like to produce ratiometric images from two stacks. The two pictures come from confocal microscope and each one of them is from different excitation wavelength.
I was trying to make it with the calculator plus tool (divide), but I got image with 0,1,2 values and not continues values.
In the next step I would like to have colors that represent the ratio values and scaling bar

Thanks for your help
Shilo