save selection of 4 different channels

[melkor2.0]

Hi everyone, I have stacks of images with 4 different channels (the 4th is
opened in grey) and I want to make a selection to copy and paste in a new
image, conserving the 4 channels. The problem is that if I work with these
multi-tiff or similar when I make a selection I only copy the present
channel, so in order to copy all at the same time I used to save in .png the
focal plane of interest, and there make the selection. However when I do
that saves the image in RGB so when my macro separates channels it will be a
mess...
any idea how to save a selection keeping the 4 channels?

Thanks,

ramon

[Mario Faretta]

Hi Ramon,
did you try the "Duplicate" command? If you select "entire stack" you
will get a new stack covering  the selected region only
Hope it helps
Mario
Il 19/06/2011 11:49, Ramon ha scritto:

> Hi everyone, I have stacks of images with 4 different channels (the 4th is
> opened in grey) and I want to make a selection to copy and paste in a new
> image, conserving the 4 channels. The problem is that if I work with these
> multi-tiff or similar when I make a selection I only copy the present
> channel, so in order to copy all at the same time I used to save in .png the
> focal plane of interest, and there make the selection. However when I do
> that saves the image in RGB so when my macro separates channels it will be a
> mess...
> any idea how to save a selection keeping the 4 channels?
>
> Thanks,
>
> ramon

[melkor2.0]

Hi Mario, thanks for the idea, but doesn't work :(  I'm doing a selection a
little bit different... I make a ROI following a shape, then enlarge the
selection and add it to the roi manager, then XOR between them and thus I
get a "donut" selection. Duplicating the selection, doesn't get only the
image between the inner and outer circles, but a rectangular selection which
compress all the outer ring
thanks,
ramon

On 19 June 2011 12:04, Mario Faretta <[hidden email]>wrote:

> Hi Ramon,
> did you try the "Duplicate" command? If you select "entire stack" you will
> get a new stack covering  the selected region only
> Hope it helps
> Mario
> Il 19/06/2011 11:49, Ramon ha scritto:
>
>> Hi everyone, I have stacks of images with 4 different channels (the 4th is
>>
>> opened in grey) and I want to make a selection to copy and paste in a new
>> image, conserving the 4 channels. The problem is that if I work with these
>> multi-tiff or similar when I make a selection I only copy the present
>> channel, so in order to copy all at the same time I used to save in .png
>> the
>> focal plane of interest, and there make the selection. However when I do
>> that saves the image in RGB so when my macro separates channels it will be
>> a
>> mess...
>> any idea how to save a selection keeping the 4 channels?
>>
>> Thanks,
>>
>> ramon
>>
>

[Gabriel Landini]

On Sunday 19 Jun 2011, Ramon <[hidden email]> wrote:
> Hi Mario, thanks for the idea, but doesn't work :(  I'm doing a selection a
> little bit different... I make a ROI following a shape, then enlarge the
> selection and add it to the roi manager, then XOR between them and thus I
> get a "donut" selection. Duplicating the selection, doesn't get only the
> image between the inner and outer circles, but a rectangular selection
> which compress all the outer ring

I think you are confused between "selection" and "image".
Images are always rectangular. What sort of value do you expect outside the
selection? A fixed colour, let's say black? If so then take the selection,
Make Inverse, fill it, Make Inverse again and finally duplicate.
Currently you cannot save an arbitrary shaped image.

Cheers
G.

[melkor2.0]

Yeah, I didn't think about the make inverse, it works fine
Thanks,

cheers

On 19 June 2011 12:21, Gabriel Landini <[hidden email]> wrote:

> On Sunday 19 Jun 2011, Ramon <[hidden email]> wrote:
> > Hi Mario, thanks for the idea, but doesn't work :(  I'm doing a selection
> a
> > little bit different... I make a ROI following a shape, then enlarge the
> > selection and add it to the roi manager, then XOR between them and thus I
> > get a "donut" selection. Duplicating the selection, doesn't get only the
> > image between the inner and outer circles, but a rectangular selection
> > which compress all the outer ring
>
> I think you are confused between "selection" and "image".
> Images are always rectangular. What sort of value do you expect outside the
> selection? A fixed colour, let's say black? If so then take the selection,
> Make Inverse, fill it, Make Inverse again and finally duplicate.
> Currently you cannot save an arbitrary shaped image.
>
> Cheers
> G.
>