No, by unchecking this ou keep the same range but only displaying between 0 - 255. As most (or all) of your intensities are above 255 the image will be white.
Kees
________________________________________
From: ImageJ Interest Group [
[hidden email]] On Behalf Of samjlord [
[hidden email]]
Sent: 21 September 2012 09:01
To:
[hidden email]
Subject: scale when converting not full range?
In Fiji, I recently tried converting a 16-bit image to 8-bit after unchecking
"scale when converting" in Edit->Options->Conversion. I expected a nearly
black image, because I thought it would rescale the entire 0-65535 range to
0-255. Instead, I got a completely white image. The image looked good when
displayed from ~350-2000, so I expected a black image when I rescaled the
full range to 0-255.
I thought that unchecking "scale when converting" meant that the entire
0-65535 range would be "rebinned" into only 0-255 gray tones. If that
happened, then the image I had should appear almost completely black, with
the 300-2000 range ending up mostly near zero when rebinned into only 256
bins. So why does the image appear white, with pixels only at 255?
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