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hi everybody!
i´m sorry to go again with the same kind of questions, but there so
many things i would like to understand...
i´m trying to quantify the colocalization of two proteins that are in
many different locations in the cell (i use primary cultures of
hepatocytes, growing on slides, i fix them and make
immunocytochemistry). I use "colocalisation thresholds" plugin, but i
run background substraction before. I realized that depending how many
times i run background substraction, the coefficients i get for the
colocalization are different.
What am i really doing when i substract background? i already read
what is available about the rolling ball, but... i´m afraid i don´t
get a real idea of the disadvantages (if any) it has, and how can it
change the values i get, and, more important, what is correct and what
is not!!
If any of you could help me...
Thank you!
Itsaso Garcia-Arcos
P.S: do you know any course on image analysis, or any good book for
self learning??
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Itsaso Garcia-Arcos
Department of Physiology, Medical School,
University of the Basque Country. UPV/EHU
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