Hi Rona,
I have not tried this myself and am new to ImageJ and imaging
but I think that this might be a job for "Skeletonization"
This would create a skeloton for each cell,
the round cells would have a simple skeleton,
the ones with small branches would have a
skeleton with small branches and the highly branched cells
would have highly branched skeletons.
There is at least one plugin for skeleton analysis
including this one:
http://imagejdocu.tudor.lu/doku.php?id=plugin:analysis:analyzeskeleton:startLooks like you could use number of branches,
average length of branches and maximum length of braches
to achieve separation of your 3 cell types.
Maybe someone else would have better advice about the details
but I think this is a good place to start
Best
Doug
rona baron wrote:
> Hi,
>
> In the system I work with, there are round cells, some cells with small
> branches and others which are highly branched.
> I want to express a quantitative differences between these morphologies.
> I thought that the relationship between the volume to the surface area of
> each object would give me this measure. (The more elaborate the object, the
> smaller the ratio).
> Do you think this calculation is right? or I need to count and measure the
> length of the branches?
>
> Thanks,
> Rona
>
>
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