I was glad to read the following in a Nature Methods special article
(the interesting part is at the end):
Nature Methods 6, 15 - 18 (2009)
Published online: 17 December 2008 | doi:10.1038/nmeth.f.234
Super-resolution microscopy: breaking the limits
Kelly Rae Chi
"...
Also collaborating with Betzig's group, a group led by Nicholas Barry,
an assistant professor of medicine at the University of
Colorado Denver, outfitted a commercial Zeiss microscope designed for
total internal reflection fluorescence (TIRF) imaging
to study how proteins cluster on the apical membranes of kidney cells.
With Zeiss's system and an average computer in hand, the assembly of
extra parts was relatively simple, Barry says. They added two lasers
at an estimated cost of $30,000.They can assemble an image, using
about ten molecules per frame, at 10,000 frames in about eight
minutes. The file size is about a third of a gigabyte. They wrote
their own software using Perl, a free programming language. "There's a
lot of background information [in the single molecule literature] you
can use to help calculate a PALM image," he says. Barry bets someone
will soon write an algorithm to run as a plug-in in ImageJ, a free
image analysis program from the National Institutes of Health. "It's
almost certain," he says.
..."
On Fri, Feb 6, 2009 at 15:29, Colin Rickman <
[hidden email]> wrote:
>
> Hi,
>
> Has anybody tried analysing PALM data using ImageJ? Is there a plugin?
>
> Colin
>
> --
> Dr Colin Rickman
> Centre for Integrative Physiology
> School of Biomedical Sciences
> University of Edinburgh
> Hugh Robson Building
> George Square
> Edinburgh
> EH8 9XD
>
> Tel: +44 131 6511512
> Fax: +44 131 6503128
>
>
> The University of Edinburgh is a charitable body, registered in
> Scotland, with registration number SC005336.
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