Import your time series of images as a sequence stack into ImageJ
(File->Import image sequence).
Draw a region of interest box on the desired location of your image
on one of the images in the stack.
To plot the average pixel intensity in in the region of interest as a
function of time, choose the Image->Stacks->Plot z-axis profile
function.
The graph can be exported as a text file for Excel spread sheets if
desired.
The same operation can be performed on a single pixel if you choose
the point-picker tool instead of the region of interest drawing tool.
John Oreopoulos, BSc,
PhD Candidate
University of Toronto
Institute For Biomaterials and Biomedical Engineering
Centre For Studies in Molecular Imaging
On 9-Feb-09, at 10:12 AM, Larsen, Henning Engelbrecht wrote:
> I am evaluating ImageJ and I welcome any hints on the following:
>
> "How useful is ImageJ in analyzing a series of images (a movie) with
> respect to the time evolution?"
>
> Typically I want to evaluate how the average intensity value (in gray
> scale) of a region evolves from image to image and make a plot of this
> evolution.
>
> I would appreciate any advices as to the suitability of ImageJ in that
> respect or if this functionality is beyond its primary scope.
>
> Thanks,
> henning
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