Hello, I was wondering if anyone could help me figure out a way to take the average pixel intensity along a high number of concentric circles (~100).
I know the Concentric Circle plugin will let me overlay concentric circles on the image... but is there anyway to get the average intensity along/around the perimeter of each of these circles? Thank you! |
Convert each circle to a band one pixel wide and measure.
=========================================================================== Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU Langone Medical Center Cell: 914-309-3270 Lab: 212-263-3208 http://ocs.med.nyu.edu/microscopy & http://www.med.nyu.edu/skirball-lab/dustinlab/ -----Original Message----- From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of moxed Sent: Monday, January 20, 2014 4:33 PM To: [hidden email] Subject: Average intensity along concentric circles Hello, I was wondering if anyone could help me figure out a way to take the average pixel intensity along a high number of concentric circles (~100). I know the Concentric Circle plugin will let me overlay concentric circles on the image... but is there anyway to get the average intensity along/around the perimeter of each of these circles? Thank you! -- View this message in context: http://imagej.1557.x6.nabble.com/Average-intensity-along-concentric-circles-tp5006193.html Sent from the ImageJ mailing list archive at Nabble.com. -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html |
If your bands are too narrow, you may fall foul of significant aliasing errors, i.e. having a non-negligble number of pixels counted in more than one band.
You are welcome to use the Domain Analysys macros if they are of any use to you http://www.dsuk.biz/DSUK/ImageJ_Support.html On 21 Jan 2014, at 14:52, "Cammer, Michael" <[hidden email]> wrote: > Convert each circle to a band one pixel wide and measure. > > =========================================================================== > Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU Langone Medical Center > Cell: 914-309-3270 Lab: 212-263-3208 > http://ocs.med.nyu.edu/microscopy & http://www.med.nyu.edu/skirball-lab/dustinlab/ > > > -----Original Message----- > From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of moxed > Sent: Monday, January 20, 2014 4:33 PM > To: [hidden email] > Subject: Average intensity along concentric circles > > Hello, I was wondering if anyone could help me figure out a way to take the average pixel intensity along a high number of concentric circles (~100). > > I know the Concentric Circle plugin will let me overlay concentric circles on the image... but is there anyway to get the average intensity along/around the perimeter of each of these circles? > > Thank you! > > > > -- > View this message in context: http://imagej.1557.x6.nabble.com/Average-intensity-along-concentric-circles-tp5006193.html > Sent from the ImageJ mailing list archive at Nabble.com. > > -- > ImageJ mailing list: http://imagej.nih.gov/ij/list.html > > ------------------------------------------------------------ > This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. > ================================= > > -- > ImageJ mailing list: http://imagej.nih.gov/ij/list.html -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html |
In reply to this post by moxed
Dear non-name,
did you have a look at the IJ-plugin "Radial_Profile_Angle_Ext" that can be downloaded from: <http://imagej.nih.gov/ij/plugins/radial-profile-ext.html> The number of circles depends on the maximum diameter and you may extract the exact values of the diameters and integral values from the plot by clicking either "List" or "Save". HTH Herbie ::::::::::::::::::::::::::::::: On 20.01.14 22:32, moxed wrote: > Hello, I was wondering if anyone could help me figure out a way to > take the average pixel intensity along a high number of concentric > circles (~100). > > I know the Concentric Circle plugin will let me overlay concentric > circles on the image... but is there anyway to get the average > intensity along/around the perimeter of each of these circles? > > Thank you! -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html |
In reply to this post by moxed
On Jan 20, 2014, at 4:32 PM, moxed wrote:
> Hello, I was wondering if anyone could help me figure out a way to take the > average pixel intensity along a high number of concentric circles (~100). > > I know the Concentric Circle plugin will let me overlay concentric circles > on the image... but is there anyway to get the average intensity > along/around the perimeter of each of these circles? There is an updated version of the Concentric Circle plugin at http://rsbweb.nih.gov/ij/plugins/concentric-circles.html with a "Measure" option that calculates and displays the average intensity along the perimeter of each circle. -wayne -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html |
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