Colocalization analysis

[Adam Mace]

Hi, I am just wondering if there is any way to quantify colocalization between to IF stains when one antigen is nuclear and the other is cytoplasmic?  So far I have only been able to use the ImageJ plugins I have found for two nuclear or two cytoplasmic stains.  Any help is much appreciated!

-Adam Mace

[Robert Baer]

On 5/31/2013 4:37 PM, Adam Mace wrote:
> Hi, I am just wondering if there is any way to quantify colocalization
> between to IF stains when one antigen is nuclear and the other is
> cytoplasmic?  So far I have only been able to use the ImageJ plugins I have
> found for two nuclear or two cytoplasmic stains.  Any help is much
> appreciated!
Maybe you could clarify what it is you wish to do.  If one stain is only
in the nucleus and the other is only in the cytoplasm, by definition the
co-localization is zero.


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Robert W. Baer, Ph.D.
Professor of Physiology
Kirksille College of Osteopathic Medicine
A. T. Still University of Health Sciences
Kirksville, MO 63501 USA

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