Counting connected Droplets

[Michael]

Hello,

I would like to count and measure droplets.
Unfortunately all instructions i found so far didn't work since the drops are connected and therefore are not recognized as different objects, or the brighter middle of the drops causes problems.
Example image:


Can someone please help me?

Thank You!

Michael

[Graeme Ball-2]

maybe something like this macro?

run("8-bit");
setAutoThreshold("Triangle stack");
setThreshold(0, 251);
setOption("BlackBackground", true);
run("Convert to Mask");
run("Watershed");
run("Analyze Particles...", "size=300-750 circularity=0.3-1.00
show=Nothing display exclude clear include add");
roiManager("Show All with labels");
run("Summarize");

On Mon, Aug 5, 2013 at 11:27 PM, Michael <[hidden email]> wrote:

> Hello,
>
> I would like to count and measure droplets.
> Unfortunately all instructions i found so far didn't work since the drops
> are connected and therefore are not recognized as different objects, or the
> brighter middle of the drops causes problems.
> Example image:
> <http://imagej.1557.x6.nabble.com/file/n5004315/fc2_save_2013-07-10-140011-0000.png>
>
> Can someone please help me?
>
> Thank You!
>
> Michael
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.com/Counting-connected-Droplets-tp5004315.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[carlo bianco]

hello
I tried this command

run("8-bit");
setAutoThreshold("Default");
//run("Threshold...");
setThreshold(0, 247);

run("Watershed");

run("Analyze Particles...", "size=0-Infinity circularity=0.00-1.00 show=Nothing display clear include summarize record add in_situ");

 after a normal thresholding I run watershed, and analyze particles. These lines can be run in a batch mode, allowing you to
analyze folders of image and evaluate processed images and sumary of results. Below the result output of imagej


example-watershed.jpg    331    167469.000    505.949    83.734    84.269    26.628    23.651    108.911    0.862    0.939    NaN

best wishes

carlo bianco



________________________________
 Da: Michael <[hidden email]>
A: [hidden email]
Inviato: Martedì 6 Agosto 2013 0:27
Oggetto: Counting connected Droplets
 

Hello,

I would like to count and measure droplets.
Unfortunately all instructions i found so far didn't work since the drops
are connected and therefore are not recognized as different objects, or the
brighter middle of the drops causes problems.
Example image:
<http://imagej.1557.x6.nabble.com/file/n5004315/fc2_save_2013-07-10-140011-0000.png>

Can someone please help me?

Thank You!

Michael



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View this message in context: http://imagej.1557.x6.nabble.com/Counting-connected-Droplets-tp5004315.html
Sent from the ImageJ mailing list archive at Nabble.com.

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