Densitometry

[Van Hoomissen, Jacqueline]

Greetings,

 

I'm new to ImageJ and I have one, probably very simple question.

 

Does anyone know how to change the "direction" of the unsigned integers
(0 to 65, 535) when using Image J for densitometry of x-ray films?

 

I've scanned my x-ray film using a Microtek Scan Maker 1000XL scanner as
a 16-bit grayscale image (saved as a TIFF file) and then imported the
images into the IMAGE J software.  

 

Now when I analyze the sections the "darker" images on the film are
being assigned low integers and the "lighter" images on the film are
reading higher integers. I'd like IMAGE J to convert this so that the
darker the image the higher the integer assigned.

 

 

Thanks for your help

 

Jacqueline D. Van Hoomissen, Ph.D.

Department of Biology

University of Portland

5000 North Willamette Boulevard

Portland, Oregon 97203 USA

503-943-7779 (Office)

503-943-7123 (Science Office)

503-943-7784 (Fax)

[hidden email]

www.up.edu

 

[David Hovis]

Image -> Lookup Tables -> Invert LUT  should do what you want.

--David

----------------------------------------
David Hovis
Senior Research Associate
Department of Materials Science
Case Western Reserve University
[hidden email]

On Oct 16, 2007, at 1:33 PM, Van Hoomissen, Jacquie wrote:

> Greetings,
>
>
>
> I'm new to ImageJ and I have one, probably very simple question.
>
>
>
> Does anyone know how to change the "direction" of the unsigned  
> integers
> (0 to 65, 535) when using Image J for densitometry of x-ray films?
>
>
>
> I've scanned my x-ray film using a Microtek Scan Maker 1000XL  
> scanner as
> a 16-bit grayscale image (saved as a TIFF file) and then imported the
> images into the IMAGE J software.
>
>
>
> Now when I analyze the sections the "darker" images on the film are
> being assigned low integers and the "lighter" images on the film are
> reading higher integers. I'd like IMAGE J to convert this so that the
> darker the image the higher the integer assigned.
>
>
>
>
>
> Thanks for your help
>
>
>
> Jacqueline D. Van Hoomissen, Ph.D.
>
> Department of Biology
>
> University of Portland
>
> 5000 North Willamette Boulevard
>
> Portland, Oregon 97203 USA
>
> 503-943-7779 (Office)
>
> 503-943-7123 (Science Office)
>
> 503-943-7784 (Fax)
>
> [hidden email]
>
> www.up.edu
>
>

[Joel Sheffield]

This, in fact will not change the values of the pixels, just the
appearance.  What you need to do us use Edit>Invert to change the
values so that they go in the right direction.

Joel

Date sent:       Tue, 16 Oct 2007 14:01:10 -0400
Send reply to:   ImageJ Interest Group <[hidden email]>
From:           David Hovis <[hidden email]>
Subject:         Re: Densitometry
To:             [hidden email]

> Image -> Lookup Tables -> Invert LUT  should do what you want.
>
> --David
>
> ----------------------------------------
> David Hovis
> Senior Research Associate
> Department of Materials Science
> Case Western Reserve University
> [hidden email]
>
> On Oct 16, 2007, at 1:33 PM, Van Hoomissen, Jacquie wrote:
>
> > Greetings,
> >
> >
> >
> > I'm new to ImageJ and I have one, probably very simple question.
> >
> >
> >
> > Does anyone know how to change the "direction" of the unsigned  
> > integers
> > (0 to 65, 535) when using Image J for densitometry of x-ray films?
> >
> >
> >
> > I've scanned my x-ray film using a Microtek Scan Maker 1000XL  
> > scanner as
> > a 16-bit grayscale image (saved as a TIFF file) and then imported the
> > images into the IMAGE J software.
> >
> >
> >
> > Now when I analyze the sections the "darker" images on the film are
> > being assigned low integers and the "lighter" images on the film are
> > reading higher integers. I'd like IMAGE J to convert this so that the
> > darker the image the higher the integer assigned.
> >
> >
> >
> >
> >
> > Thanks for your help
> >
> >
> >
> > Jacqueline D. Van Hoomissen, Ph.D.
> >
> > Department of Biology
> >
> > University of Portland
> >
> > 5000 North Willamette Boulevard
> >
> > Portland, Oregon 97203 USA
> >
> > 503-943-7779 (Office)
> >
> > 503-943-7123 (Science Office)
> >
> > 503-943-7784 (Fax)
> >
> > [hidden email]
> >
> > www.up.edu
> >
> >

--
Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
[hidden email]  
[phone], fax [phone]
http://astro.temple.edu/~jbs

[Toby Cornish]

Even better, you can do both:

 Edit | Invert  to change the values, then
 
 Image | Lookup Tables | Invert LUT  to make the image look "normal" again

toby

Toby C. Cornish, M.D., Ph.D.
Pathology Resident
Johns Hopkins Medical Institutions
[hidden email]