Rudolf Oldenbourg send me his java code to de-interleave files opened
with ICS_Opener. I added his code to the ICS opener plugin, which now should automatically recognize whether a file is deinterleaved and the plugin opened the few NIkon C1 images I had quite nicely. I haven't had time to test it extensively (I don't have a C1; actually, I no longer work with ICS files at all), so please give it a try and let me know if there are problems. Download at: http:// valelab.ucsf.edu/~nico/ijplugins/Ics_Opener.html All praise for the Nikon support goes to Rudolf, I'll take complaints and bug reports. B.t.w., Chris, I didn't follow matters closely, so maybe the BioFormats plugin already opens the Nikon C1 ICS files? Best, Nico Stuurman Vale Lab, UCSF MC2240 HHMI / Dept. of Cellular and Molecular Pharmacology University of California, San Francisco Genentech Hall, Room N316 600 16th street San Francisco, CA 94158-2517 |
Hi Nico,
I do not believe we have any Nikon C1 ICS files, so it is unlikely that Bio-Formats supports them. But feel free to give it a try. If they don't work, we would be glad to look into adding support if you could send us some sample data. I'm sure your updated ICS_Opener plugin would be a good resource, as well. Thanks, Curtis On 2/16/07, Nico Stuurman <[hidden email]> wrote: > Rudolf Oldenbourg send me his java code to de-interleave files opened > with ICS_Opener. I added his code to the ICS opener plugin, which > now should automatically recognize whether a file is deinterleaved > and the plugin opened the few NIkon C1 images I had quite nicely. I > haven't had time to test it extensively (I don't have a C1; actually, > I no longer work with ICS files at all), so please give it a try and > let me know if there are problems. Download at: http:// > valelab.ucsf.edu/~nico/ijplugins/Ics_Opener.html > > All praise for the Nikon support goes to Rudolf, I'll take complaints > and bug reports. > > B.t.w., Chris, I didn't follow matters closely, so maybe the > BioFormats plugin already opens the Nikon C1 ICS files? > > Best, > > > Nico Stuurman > Vale Lab, UCSF MC2240 > HHMI / Dept. of Cellular and Molecular Pharmacology > University of California, San Francisco > Genentech Hall, Room N316 > 600 16th street > San Francisco, CA 94158-2517 > |
Couldn't open interleaved .ids stacks. *shrugs*
With the "old" ICS opener version, I just did File->Import->Image Sequence and open up one .ids and all of them opened just fine. The only up-side to having a deinterleaving ics opener plugin is to cut down on files (especially for spectral). When saved, there are 8 files (ics/ids for our 3 channels, an extra .ics and a .isr that puts them back together in NotSo-EZC1) versus 2 files in an interleaved file. 4x the amount of files written to disk, so I guess it could help fragment hard drives. I'm currently using EZ-C1 3.2 Build 610. I need to update to EZ-C1 3.30. Griffin Perry Research Assistant Department of Endodontics, Dental School University of Texas Health Science Center 7703 Floyd Curl Drive Mail Code 7892 San Antonio, TX 78229-3900 Lab: (210) 567-0898 FAX (210) 567-3389 E-mail: [hidden email] -----Original Message----- From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Curtis Rueden Sent: Friday, February 16, 2007 12:19 PM To: [hidden email] Subject: Re: ICS_opener release with Nikon C1 support Hi Nico, I do not believe we have any Nikon C1 ICS files, so it is unlikely that Bio-Formats supports them. But feel free to give it a try. If they don't work, we would be glad to look into adding support if you could send us some sample data. I'm sure your updated ICS_Opener plugin would be a good resource, as well. Thanks, Curtis On 2/16/07, Nico Stuurman <[hidden email]> wrote: > Rudolf Oldenbourg send me his java code to de-interleave files opened > with ICS_Opener. I added his code to the ICS opener plugin, which > now should automatically recognize whether a file is deinterleaved > and the plugin opened the few NIkon C1 images I had quite nicely. I > haven't had time to test it extensively (I don't have a C1; actually, > I no longer work with ICS files at all), so please give it a try and > let me know if there are problems. Download at: http:// > valelab.ucsf.edu/~nico/ijplugins/Ics_Opener.html > > All praise for the Nikon support goes to Rudolf, I'll take complaints > and bug reports. > > B.t.w., Chris, I didn't follow matters closely, so maybe the > BioFormats plugin already opens the Nikon C1 ICS files? > > Best, > > > Nico Stuurman > Vale Lab, UCSF MC2240 > HHMI / Dept. of Cellular and Molecular Pharmacology > University of California, San Francisco > Genentech Hall, Room N316 > 600 16th street > San Francisco, CA 94158-2517 > |
> Couldn't open interleaved .ids stacks. *shrugs*
Instead of shrugging you could: 1. Use the included source code to fix the problem and submit the fix to the plugin's author 2. Send a comprehensible bug report to the plugin's author (including a small example of the problematic files). Option #2 has gotten a bit harder since shrugging leads to automatic addition of your email address to my spam filters. Nico > With the "old" ICS opener version, I just did File->Import->Image > Sequence and open up one .ids and all of them opened just fine. The > only up-side to having a deinterleaving ics opener plugin is to cut > down > on files (especially for spectral). When saved, there are 8 files > (ics/ids for our 3 channels, an extra .ics and a .isr that puts them > back together in NotSo-EZC1) versus 2 files in an interleaved > file. 4x > the amount of files written to disk, so I guess it could help fragment > hard drives. > > I'm currently using EZ-C1 3.2 Build 610. I need to update to EZ-C1 > 3.30. > > Griffin Perry > Research Assistant > Department of Endodontics, Dental School > University of Texas Health Science Center > 7703 Floyd Curl Drive Mail Code 7892 > San Antonio, TX 78229-3900 > Lab: (210) 567-0898 FAX (210) 567-3389 > E-mail: [hidden email] > > -----Original Message----- > From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of > Curtis Rueden > Sent: Friday, February 16, 2007 12:19 PM > To: [hidden email] > Subject: Re: ICS_opener release with Nikon C1 support > > Hi Nico, > > I do not believe we have any Nikon C1 ICS files, so it is unlikely > that Bio-Formats supports them. But feel free to give it a try. If > they don't work, we would be glad to look into adding support if you > could send us some sample data. I'm sure your updated ICS_Opener > plugin would be a good resource, as well. > > Thanks, > Curtis > > On 2/16/07, Nico Stuurman <[hidden email]> wrote: >> Rudolf Oldenbourg send me his java code to de-interleave files opened >> with ICS_Opener. I added his code to the ICS opener plugin, which >> now should automatically recognize whether a file is deinterleaved >> and the plugin opened the few NIkon C1 images I had quite nicely. I >> haven't had time to test it extensively (I don't have a C1; actually, >> I no longer work with ICS files at all), so please give it a try and >> let me know if there are problems. Download at: http:// >> valelab.ucsf.edu/~nico/ijplugins/Ics_Opener.html >> >> All praise for the Nikon support goes to Rudolf, I'll take complaints >> and bug reports. >> >> B.t.w., Chris, I didn't follow matters closely, so maybe the >> BioFormats plugin already opens the Nikon C1 ICS files? >> >> Best, >> >> >> Nico Stuurman >> Vale Lab, UCSF MC2240 >> HHMI / Dept. of Cellular and Molecular Pharmacology >> University of California, San Francisco >> Genentech Hall, Room N316 >> 600 16th street >> San Francisco, CA 94158-2517 >> |
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