Making your threshold the ROI

[Perry, Griffin M]

Dear ImageJ Enthusiasts and Part-Timers,

 

Once I threshold a grayscale image, how can I select the thresholded
area(s) as a ROI(s).  Is there an easier way than selecting each one
individually?  I'm dealing with 1000's of punctate ROIs and any help
would be appreciated.

 

-Griffin Perry

UTHSCSA

 

[jmutterer]

You can use the Edit>Selection>Create Selection menu command.

Jerome

On 9/5/06, Perry, Griffin M <[hidden email]> wrote:

> Dear ImageJ Enthusiasts and Part-Timers,
>
>
>
> Once I threshold a grayscale image, how can I select the thresholded
> area(s) as a ROI(s).  Is there an easier way than selecting each one
> individually?  I'm dealing with 1000's of punctate ROIs and any help
> would be appreciated.
>
>
>
> -Griffin Perry
>
> UTHSCSA
>
>
>

[Soft Aliance]

Use analyze particles with record starts option. After that you can use
either the Add Particles option in MultiMeasure plugin or Add Particles
option of the Latest ROI Manager (ImageJ version 1.37q). Also you can search
the ImageJ List to get a macro that Wayne wrote for someone.

On 9/5/06, Perry, Griffin M <[hidden email]> wrote:

>
> Dear ImageJ Enthusiasts and Part-Timers,
>
>
>
> Once I threshold a grayscale image, how can I select the thresholded
> area(s) as a ROI(s).  Is there an easier way than selecting each one
> individually?  I'm dealing with 1000's of punctate ROIs and any help
> would be appreciated.
>
>
>
> -Griffin Perry
>
> UTHSCSA
>
>
>

--
Balaji J
Department of biochemistry
Weil Medical College of Cornell University, New York - 113 74.
alt email: [hidden email]

[Perry, Griffin M]

So I have found Wayne's macro ROIManagerAddParticles_.txt.  However, it
wants to run("Blobs (25K)").  How would I edit this to run(active
window)?

Fortran trained, Java inept,

Griffin Perry

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
Perry, Griffin M
Sent: Tuesday, September 05, 2006 3:45 PM
To: [hidden email]
Subject: Making your threshold the ROI

Dear ImageJ Enthusiasts and Part-Timers,

 

Once I threshold a grayscale image, how can I select the thresholded
area(s) as a ROI(s).  Is there an easier way than selecting each one
individually?  I'm dealing with 1000's of punctate ROIs and any help
would be appreciated.

 

-Griffin Perry

UTHSCSA