Hi All,
I am new to ImageJ, and am trying to figure out how to measure the volumes of corrosion pits on polished metal surfaces.
The raw data consists of image stacks collected with a laser confocal microscope, which are readily read and interpreted by ImageJ. The surface is defined by the layer with the brightest intensity. I need to be able to correct of surface tilt, determine the elevation in the stack for each x,y point, interpolate between adjacent layers to fill in holes (sometimes the pits walls are too steep, and leave shadows in the pit), and then calculate the pit volume and area. I think I can work out the individual steps if I have to, but suspect that the macros have already been developed, since this seems like a standard type of analysis. Does anyone have any suggestions?
Thanks,
Charles Bryan
[hidden email]
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