Hi!
I am not an expert, so maybe there is a better solution to do it, but
I am doing exactly the same, measuring volume of dendrites and axons
and the way I do it (to be honest is good but a bit time consuming) is
to :
1. Using the segmentation editor plug-in I create the volume of the
dendrites (If I have confocal channels of different colours I do this
twice one with each channel)
2. With the tool threshold I convert the new stack(s) into a binary
file.
3. Using the plug in colour counting gives me the pixel of each of the
colors (that I can look at pixels of overlap as well)
4. I can transform the pixels into µm3 because I know my pixel size.
Hope that helps, and if you find a better or faster way of doing it,
please let me know,
Best,
Lucia
On 27 Feb 2009, at 22:12, Bin Ma wrote:
> hello everyone,
> does anyone have idea how to
> 1. segment the neuron dendrites or axons and get the area of
> it( pixels or
> in um2)
> 2. How to get the volume of the dendrites using image stack of optic
> sections from microscopy?
> thank you.
> Bin Ma MD, PhD
> NYU school of Medicine
Lucia Prieto Godino
PhD student.
Department of Zoology,
Downing street
University of Cambridge.
UK
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