Pulling frames from large movies in QT (OSX)?

[Steve Nanchy]

Is there a plugin I can use within a macro to pull individual frames (or a range of frames) from a Quicktime movie (running OSX 10.4.11 here)?
I need to recursively search rather large (100-200meg) movies that are just TOO big to pull into ImageJ.
I see I can create a 'virtual stack' when opening an image sequence, but nothing to do the same with a movie...
Thanks.


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[Roger Koot]

You might be helped with ffmpeg. Use ffmpeg to convert the qt files to
images which you can use in imagej

Hope this helps

Roger


On 12/10/2009 03:52 PM, Nanchy III, Stephen wrote:
> Is there a plugin I can use within a macro to pull individual frames (or a range of frames) from a Quicktime movie (running OSX 10.4.11 here)?
> I need to recursively search rather large (100-200meg) movies that are just TOO big to pull into ImageJ.
> I see I can create a 'virtual stack' when opening an image sequence, but nothing to do the same with a movie...
> Thanks.
>
>
> ________________________________
> This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the named addressee you should not disseminate, distribute, retain, or copy this e-mail or any attachments. If you have received this email in error please delete and notify the sender.

[Knecht, David]

You can export the movie from Quicktime player as an image sequence  
(Export/movie to image sequence) and then open subsets of frames in  
ImageJ for analysis or use the Virtual stack.  Dave

On Dec 10, 2009, at 10:21 AM, Roger Koot wrote:

> You might be helped with ffmpeg. Use ffmpeg to convert the qt files to
> images which you can use in imagej
>
> Hope this helps
>
> Roger
>
>
> On 12/10/2009 03:52 PM, Nanchy III, Stephen wrote:
>> Is there a plugin I can use within a macro to pull individual  
>> frames (or a range of frames) from a Quicktime movie (running OSX  
>> 10.4.11 here)?
>> I need to recursively search rather large (100-200meg) movies that  
>> are just TOO big to pull into ImageJ.
>> I see I can create a 'virtual stack' when opening an image  
>> sequence, but nothing to do the same with a movie...
>> Thanks.
>>
>>
>> ________________________________
>> This email and any files transmitted with it are confidential and  
>> intended solely for the use of the individual or entity to whom  
>> they are addressed. If you are not the named addressee you should  
>> not disseminate, distribute, retain, or copy this e-mail or any  
>> attachments. If you have received this email in error please delete  
>> and notify the sender.

Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)

[Wayne Rasband]

 > Is there a plugin I can use within a macro to pull
 > individual frames (or a range of frames) from a Quicktime
 > movie (running OSX 10.4.11 here)? I need to recursively
 > search rather large (100-200meg) movies that are just TOO
 > big to pull into ImageJ. I see I can create a 'virtual
 > stack' when opening an image sequence, but nothing to do
 > the same with a movie... Thanks.

The latest version of the QT_Movie_Opener plugin (File>Import>Using
QuickTime command) at

    http://rsb.info.nih.gov/ij/plugins/movie-opener.html

supports virtual stacks, or you can convert QuickTime movies to image
sequences using a conversion program like "MPEG Streamclip".

-wayne

[ctrueden]

Hi Stephen,

In addition to the other solutions presented, you could also use Bio-Formats
(http://www.loci.wisc.edu/bio-formats). You can open a QuickTime movie as a
virtual stack (including a subset of planes of your choice) using the
Bio-Formats Importer plugin, or you can use the Bio-Formats macro extensions
to flexibly work with individual planes (see
http://www.loci.wisc.edu/bio-formats/imagej for examples).

-Curtis

On Thu, Dec 10, 2009 at 8:52 AM, Nanchy III, Stephen <
[hidden email]> wrote:

> Is there a plugin I can use within a macro to pull individual frames (or a
> range of frames) from a Quicktime movie (running OSX 10.4.11 here)?
> I need to recursively search rather large (100-200meg) movies that are just
> TOO big to pull into ImageJ.
> I see I can create a 'virtual stack' when opening an image sequence, but
> nothing to do the same with a movie...
> Thanks.
>
>
> ________________________________
> This email and any files transmitted with it are confidential and intended
> solely for the use of the individual or entity to whom they are addressed.
> If you are not the named addressee you should not disseminate, distribute,
> retain, or copy this e-mail or any attachments. If you have received this
> email in error please delete and notify the sender.
>