Hello, folks, and thanks in advance for any help you can provide.
I have two goals associated with the image sequence found in the link at the end of this message (I'm working with a .tif stack in Fiji, but linked to the .gif for easier viewing): 1) quantify the velocity of the sand particle at incipient motion (i.e. when it transitions from a stationary to a mobile state); and 2) quantify the mass of the mobilized particle.
Regarding the first goal, I believe the challenge lies in processing the image in such a way that the particle of interest can be distinguished from the rest of the particles, but so far I've been unsuccessful at this. Can anyone recommend a particular technique?
Regarding the second goal, my first — and so far, only — idea is to define the particle's boundary in successive frames as it rolls along its path and use these measurements to establish a mean particle diameter. With knowledge of the material's density, I can calculate an estimated mass. I'm not optimistic that this will yield accurate results, and welcome any alternative and more robust ideas you folks may have.
Again, thanks in advance for any advice offered.
-Ben
https://drive.google.com/file/d/0BzjrjrvXKlvFbVdKZ1V2bkJyUms/edit?usp=sharing--
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