Yes you can do it, with a reference. There has to be a scale of the colors to use for the backwards conversion. It involves either (macro) programming or tedious sampling manually and making a conversion table. We did this years ago to get Metamorph LUTs that weren't in NIH-Image to use in NIH-Image.
I think if there are colors missing from the scale that are used in the image, it may be approximated by doing a 7, 6, 5, 4 bit color conversion. Perhaps a better programmer could come up with a better way to do this.
Michael Cammer, Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
C: 914-309-3270
[hidden email] http://microscopynotes.com/https://med.nyu.edu/research/research-resources/scientific-cores-shared-resources/microscopy-laboratory________________________________________
From: Paul Stoodley [
[hidden email]]
Sent: Tuesday, August 29, 2017 5:37 PM
To:
[hidden email]
Subject: Reverse heat map
Hello,
I have some images that have been saved with a heat map where red is most
intense and black is least in terms of bioluminescence. There is a color
bar scale but I was wondering if it is possible to convert this to grey
scale (preserving the relative intensity values) not just converting to 8
bit. Sincerely, Paul.
--
Paul Stoodley, PhD.
Director, Campus Microscopy and Imaging Facility (CMIF)
Professor, Departments of Microbial Infection and Immunity and Orthopaedics
Infectious Diseases Institute,
716 Biomedical Research Tower,
The Ohio State University, 460 West 12th Avenue, Columbus OH 43210.
+1 614 292 7826
Professor Microbial Tribology
National Centre for Advanced Tribology at Southampton (nCATS)
Dept. Mech. Eng.
Engineering Sciences
University of Southampton, SO17 1BJ. UK
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