Segmentation of phase contrast images

[Jacqueline Ross]

Dear All,

 

I'm trying to segment phase contrast images of adherent cells but I'm
having some trouble doing it. At the moment, I don't need to know how
many cells there are but simply the area covered by the cells.

 

I would appreciate any suggestions.

 

Cheers,

 

Jacqui.

 

Jacqueline Ross
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND

Tel: [phone] Ext 87438
Fax: [phone]

http://www.health.auckland.ac.nz/biru/
<http://www.health.auckland.ac.nz/biru/>  

 

[Theresa Swayne]

Hi Jacqui,

On Nov 20, 2006, at 7:02 PM, Jacqui Ross wrote:
> I'm trying to segment phase contrast images of adherent cells but I'm
> having some trouble doing it. At the moment, I don't need to know how
> many cells there are but simply the area covered by the cells.


You might try edge detection followed by filling holes.

Hope this helps,
Theresa

====================================
Theresa C. Swayne                                 [hidden email]
Pon lab/Optical Microscopy Facility     [phone]
Columbia University, New York, NY

http://cancercenter.columbia.edu/~confocal

[Dimiter Prodanov]

>Dear Jacqui,
If the question is so simple why do you need segmentation.
You can get by generating a Grid of points and then simply counting
how many crosses hit any of the cells.
In such way you ge the area estimate of the cells. then simply subtract
the area which is obviously void of cells (I mean
large empty fields) and take the ratio and voilà -
you ll have the area fraction.
I did the same while counting muscle cells
in cross sections (quite difficult to segment).

best regards


_______________________________________________________________________
Dr Dimiter Prodanov, MD, Ph.D.

Neural Engineering Rehabilitation Laboratory
(Laboratoire de Génie de la Réhabilitation Neurale)
Département de Physiologie et Pharmacologie
Université catholique de Louvain
Avenue Hippocrate, 54
POBox UCL-5446 / B-1200 Bruxelles -Belgique-
Phone: [phone]
Fax: [phone]

http://www.md.ucl.ac.be/gren