Wrong Order in Stack Building

[Gabriel Shemer]

Hello,
I use a fluorescent microscope to take images in time. Each image is saved
in 16-bit tiff format and enumerated (1,2,3....). I would like to create a
stack of theses images (more than 100). When I open all the images in ImageJ
and convert them to a stack, some of them are not positioned in their
correct place (e.g., the order of the images is 0, 1, 10, 101,
102,.....109,11).
I would appreciate any help in this matter.
Thanks,
Gabriel

[Jeffrey B. Woodward]

Pad your filenames to be 001, 002, 003, 004, ..., 999

-Woody


Gabriel Shemer wrote:

> Hello,
> I use a fluorescent microscope to take images in time. Each image is saved
> in 16-bit tiff format and enumerated (1,2,3....). I would like to create a
> stack of theses images (more than 100). When I open all the images in ImageJ
> and convert them to a stack, some of them are not positioned in their
> correct place (e.g., the order of the images is 0, 1, 10, 101,
> 102,.....109,11).
> I would appreciate any help in this matter.
> Thanks,
> Gabriel
>
>
>  

[Michael Schmid]

Hi Gabriel,

you could also try Import>Image Sequence and check "Sort names numerically".

Michael
________________________________________________________________________

 Gabriel Shemer wrote:

> Hello,
> I use a fluorescent microscope to take images in time. Each image is
> saved
> in 16-bit tiff format and enumerated (1,2,3....). I would like to create
> a
> stack of theses images (more than 100). When I open all the images in
> ImageJ
> and convert them to a stack, some of them are not positioned in their
> correct place (e.g., the order of the images is 0, 1, 10, 101,
> 102,.....109,11).
> I would appreciate any help in this matter.
> Thanks,
> Gabriel

[Wayne Rasband]

On Jan 12, 2010, at 10:34 AM, Gabriel Shemer <[hidden email]
 > wrote:

> Hello,
> I use a fluorescent microscope to take images in time. Each image is  
> saved
> in 16-bit tiff format and enumerated (1,2,3....). I would like to  
> create a
> stack of theses images (more than 100). When I open all the images  
> in ImageJ
> and convert them to a stack, some of them are not positioned in their
> correct place (e.g., the order of the images is 0, 1, 10, 101,
> 102,.....109,11).
> I would appreciate any help in this matter.
> Thanks,
> Gabriel

Drag and drop the folder containg the images onto the "ImageJ" window  
and ImageJ will open it as a correctly ordered stack.

-wayne