analyze particles

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analyze particles

Elisa Poggianti
Hi,
I'm new to the group and was wondering if anyone could help me:

I need to obtain the aspect ratio (width/lenght) of random oriented
particles of different polygonal shapes. Using "bounding rectangle", the
width and the height are wrong because of the orientation; Feret's
diameter are good measures of the long axes but I need also the width.
How can I get a real aspect ratio for each particles?
Thanks
Elisa
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Re: analyze particles

Gary Chinga
Take a look at the shape descriptor plugin.
http://www.gcsca.net/IJ/Shapes.html

Gary.


On May 7, 2006, at 3:59 PM, Elisa Poggianti wrote:

> Hi,
> I'm new to the group and was wondering if anyone could help me:
>
> I need to obtain the aspect ratio (width/lenght) of random oriented
> particles of different polygonal shapes. Using "bounding  
> rectangle", the
> width and the height are wrong because of the orientation; Feret's
> diameter are good measures of the long axes but I need also the width.
> How can I get a real aspect ratio for each particles?
> Thanks
> Elisa
>
>
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Re: analyze particles

Manuel Urrutia Avisrror
In reply to this post by Elisa Poggianti
In nuclear morphometry the aspect ratio is defined as:
MinAxes/maxAxes * 1000; values near to 1000 would
describe a perfect circle while below 1000 eliptic forms
I've written a module for Image 1.63 that works fine
with MacOSX where you can perform fully automated
particle analysis and calculates many different shape descriptors.
You may download it form the NIH server
 Best regards

Manuel Urrutua MD, PhD

----- Original Message -----
From: "Elisa Poggianti" <[hidden email]>
To: <[hidden email]>
Sent: Sunday, May 07, 2006 3:59 PM
Subject: analyze particles


Hi,
I'm new to the group and was wondering if anyone could help me:

I need to obtain the aspect ratio (width/lenght) of random oriented
particles of different polygonal shapes. Using "bounding rectangle", the
width and the height are wrong because of the orientation; Feret's
diameter are good measures of the long axes but I need also the width.
How can I get a real aspect ratio for each particles?
Thanks
Elisa