dendritic spine quantification

[Darren Scully]

Hello,

I am an extremely new user of ImageJ.  I have only downloaded it last week. So I was wondering if there is any mechanism whereby one can quantify spines protruding from a dendrite.  I will be using two mechanisms to image the dendrites and spines. Flourescent micrsoscopy to give me 2D views and also confocal microscopy.

I was wondering if I can quantify the spines using either or both techniques?  Preferably using confocal so I can obtain the real spine number and preclude double-counting of the same spine.

Many thanks in advance.

Darren

[Sébastien Thomas]

Darren,

        Look into NeuronJ, a plugin that works as a stand alone program  
that's perfectly fit for quantifying dendritic morphology.

Sébastien Thomas


Le 26-06-2006 à 07:56, Darren Scully a écrit :

> Hello,
>
> I am an extremely new user of ImageJ.  I have only downloaded it  
> last week. So I was wondering if there is any mechanism whereby one  
> can quantify spines protruding from a dendrite.  I will be using  
> two mechanisms to image the dendrites and spines. Flourescent  
> micrsoscopy to give me 2D views and also confocal microscopy.
>
> I was wondering if I can quantify the spines using either or both  
> techniques?  Preferably using confocal so I can obtain the real  
> spine number and preclude double-counting of the same spine.
>
> Many thanks in advance.
>
> Darren

[Dimiter Prodanov]

>Hi Darren,

I published an article recently on synaptic bouton counting
using ImageJ. May be it could help?
Check in PubMed for Prodanov et al. in Journal of Neuroscience Methods.

best regards
Dimiter



_______________________________________________________________________
Dr Dimiter Prodanov, MD, Ph.D.

Neural Engineering Rehabilitation Laboratory
(Laboratoire de Génie de la Réhabilitation Neurale)
Département de Physiologie et Pharmacologie
Université catholique de Louvain
Avenue Hippocrate, 54
POBox UCL-5446 / B-1200 Bruxelles -Belgique-
Phone: [phone]
Fax: [phone]

http://www.md.ucl.ac.be/gren