use of imagej to count cells?

[Owen Lockerbie]

does anyone know if image j is able to count positive vs negative stained cells in an immunohistochemical image? - i've attached below a .jpeg image as an example of tumor cells staining positive (brown) vs non-staining cells (blue). it would be great if the program can be used to mark and record # of positive cells vs negative cells instead of laborious and error -prone manual counting
 
in the help menu of the program there doesn't seem to be a counting tool, but maybe i'm just not finding it?
 
any help in using the program for this purpose would be greatly appreciated
 
many thanks!
 
 
Owen Lockerbie, PhD
University of Colorado Cancer Center
Division of Medical Oncology
Aurora CO 80010
Tel [phone]
email : [hidden email]
 
 <https://webmail.uchsc.edu/exchange/Owen.Lockerbie/Sent%20Items/No%20Subject-17.EML/Ki67%236-2%20061206.jpg/C58EA28C-18C0-4a97-9AF2-036E93DDAFB3/Ki67%236-2%20061206.jpg?attach=1>
 
 
 
 
 
 
 
 
 
 
 
Owen Lockerbie, PhD
University of Colorado Cancer Center
Division of Medical Oncology
Aurora CO 80010
Tel [phone]
email : [hidden email]

________________________________

From: Lockerbie, Owen
Sent: Mon 6/26/2006 1:34 PM
To: [hidden email]
Subject:


does anyone know if image j is able to count positive vs negative stained cells in an immunohistochemical image? - i've attached a .jpeg image as an example of tumor cells staining positive (brown) vs non-staining cells (blue). it would be great if the program can be used to mark and record # of positive cells vs negative cells instead of laborious and error -prone manual counting
 
in the help menu of the program there doesn't seem to be a counting tool, but maybe i'm just not finding it?
 
any help in using the program for this purpose would be greatly appreciated
 
many thanks!
 
 
Owen Lockerbie, PhD
University of Colorado Cancer Center
Division of Medical Oncology
Aurora CO 80010
Tel [phone]
email : [hidden email]

[Vincenzo Della Mea]

If you mean automatic evaluation, ImageJ is not doing that by itself,  
but in principle can be programmed to do it. However, it is a  
difficult task, due to the many variables involved in the staining  
process (I have a student doing a master thesis on that, and I had  
other students in the past, not with ImageJ). A little bit easier  
could be if done on tissue microarrays, because of the possibility of  
including a control core with known positivity and intensity.
By the way, your attachment cannot be reached without a password.
Regards,
Vincenzo Della Mea

Il giorno 26/giu/06, alle ore 21:39, Owen Lockerbie ha scritto:

> does anyone know if image j is able to count positive vs negative  
> stained cells in an immunohistochemical image? - i've attached  
> below a .jpeg image as an example of tumor cells staining positive  
> (brown) vs non-staining cells (blue). it would be great if the  
> program can be used to mark and record # of positive cells vs  
> negative cells instead of laborious and error -prone manual counting
>
> in the help menu of the program there doesn't seem to be a counting  
> tool, but maybe i'm just not finding it?
>
> any help in using the program for this purpose would be greatly  
> appreciated

* Vincenzo Della Mea
* Medical Informatics, Telemedicine and Ehealth Lab
* University od Udine, Italy
* http://mitel.dimi.uniud.it/  -  http://www.eslide.net

[Klughammer GmbH]

Hi,

could you send the image to my personal email address, then I will have
a look on it.

Anneliese
[hidden email]


Vincenzo Della Mea schrieb:

> If you mean automatic evaluation, ImageJ is not doing that by itself,  
> but in principle can be programmed to do it. However, it is a  
> difficult task, due to the many variables involved in the staining  
> process (I have a student doing a master thesis on that, and I had  
> other students in the past, not with ImageJ). A little bit easier  
> could be if done on tissue microarrays, because of the possibility of  
> including a control core with known positivity and intensity.
> By the way, your attachment cannot be reached without a password.
> Regards,
> Vincenzo Della Mea
>
> Il giorno 26/giu/06, alle ore 21:39, Owen Lockerbie ha scritto:
>
>> does anyone know if image j is able to count positive vs negative  
>> stained cells in an immunohistochemical image? - i've attached  below
>> a .jpeg image as an example of tumor cells staining positive  (brown)
>> vs non-staining cells (blue). it would be great if the  program can
>> be used to mark and record # of positive cells vs  negative cells
>> instead of laborious and error -prone manual counting
>>
>> in the help menu of the program there doesn't seem to be a counting  
>> tool, but maybe i'm just not finding it?
>>
>> any help in using the program for this purpose would be greatly  
>> appreciated
>
>
> * Vincenzo Della Mea
> * Medical Informatics, Telemedicine and Ehealth Lab
> * University od Udine, Italy
> * http://mitel.dimi.uniud.it/  -  http://www.eslide.net
>