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gray-color merge

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gray-color merge

Knecht, David
143 posts
I have two stacks which I would like to merge. One is the grayscale  
of the DIC image and the other is a grayscale image with a color LUT  
applied.  It needs to come as color on gray and would be best if the  
color were subtracted from the gray prior to merge so that the color  
is not washed out by the gray.   The only way I have found is to  
convert the 8 bit LUT image to RGB, split the RGB, then subtract each  
from the gray channel, then use RGB Gray Merge to merge them into a  
new RGB.  It works, but perhaps there is a better way?  Dave

Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)
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Re: gray-color merge

Tony Collins-4
39 posts
Hi Dave,
I've tweaked the RGB grey merge from the imagej website:
http://rsb.info.nih.gov/ij/plugins/rgb-gray-merge.html

to allow the subtract a user defined percentage of the fluorescent channels from the grey prior to merging.

You can get it with the MacBiophotonics ImageJ bundle or I can email it to you directly off list.

Any feedback much appreciated.

Tony

Tony J. Collins, Ph.D.
McMaster Biophotonics Facility
Dept. Biochemistry and Biomedical Sciences HSC 4H21A
McMaster University, Hamilton, ON, L8N 3Z5
(905) 525 9140 x28812(off.)/x26488(lab)
[hidden email]     www.macbiophotonics.ca


> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
> David Knecht
> Sent: Friday, February 09, 2007 2:52 PM
> To: [hidden email]
> Subject: gray-color merge
>
> I have two stacks which I would like to merge. One is the grayscale
> of the DIC image and the other is a grayscale image with a color LUT
> applied.  It needs to come as color on gray and would be best if the
> color were subtracted from the gray prior to merge so that the color
> is not washed out by the gray.   The only way I have found is to
> convert the 8 bit LUT image to RGB, split the RGB, then subtract each
> from the gray channel, then use RGB Gray Merge to merge them into a
> new RGB.  It works, but perhaps there is a better way?  Dave
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)