how to measure dimensions of middle ear structure

[m_hatzim]

Dear colleagues,

I am working on Histologic sections in temporal bones and I need to get exact measurements of the diameter of long process of incus. For every single specimen I have about 30 sections that includes incus long process. However, sections are not perpedicular to the long process. Is it possible using ImageJ to reconstruct a clear perpedicular cut to incus, so I can measure the diameter of long process? I would be grateful if I could have information about the steps of how to do it.
Best regards, Michail

[Santosh M]

hi

This has been discussed many times

go through following link

http://rsbweb.nih.gov/ij/docs/examples/dna-contours/index.html

Properly set the measurement

You have to define your ROI for each image

regards

Santosh

2010/1/27 m_hatzim <[hidden email]>:

> Dear colleagues,
>
> I am working on Histologic sections in temporal bones and I need to get
> exact measurements of the diameter of long process of incus. For every
> single specimen I have about 30 sections that includes incus long process.
> However, sections are not perpedicular to the long process. Is it possible
> using ImageJ to reconstruct a clear perpedicular cut to incus, so I can
> measure the diameter of long process? I would be grateful if I could have
> information about the steps of how to do it.
> Best regards, Michail
>
> --
> View this message in context: http://n2.nabble.com/how-to-measure-dimensions-of-middle-ear-structure-tp4467091p4467091.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>

--
शुभकामनायें/Best regards

Santosh

[Julian Cooper]

Hi Michail,

You might find the Untilt Stack plugin:
http://rsb.info.nih.gov/ij/plugins/untilt-stack/index.html
does what you need. There are detailed instructions on the plugin
documentation page.

Best wishes,

Julian

> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On
> Behalf Of m_hatzim
> Sent: 27 January 2010 13:23
> To: [hidden email]
> Subject: how to measure dimensions of middle ear structure
>
>
> Dear colleagues,
>
> I am working on Histologic sections in temporal bones and I
> need to get
> exact measurements of the diameter of long process of incus. For every
> single specimen I have about 30 sections that includes incus
> long process.
> However, sections are not perpedicular to the long process.
> Is it possible
> using ImageJ to reconstruct a clear perpedicular cut to
> incus, so I can
> measure the diameter of long process? I would be grateful if
> I could have
> information about the steps of how to do it.
> Best regards, Michail
>
> --
> View this message in context:
> http://n2.nabble.com/how-to-measure-dimensions-of-middle-ear-s

tructure-tp4467091p4467091.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>

[Albert Cardona-2]

The "Plugins - Transform - Interactive Stack Rotation" will do it:

1. Open a stack
2. Run the plugin
3. push 'F1' to see all mouse and key bindings to rotate the stack
4. when done rotating, make sure the window is active and push 'enter'
to convert it to a regular ImageJ stack.
5. Draw a line ROI and push 'm' to measure it.

It's in Fiji:  http://pacific.mpi-cbg.de

Albert
--
http://albert.rierol.net

[Peter Stevrin]

Is it also possible to measure the dimensions of the ear canal?

/Peter

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Albert
Cardona
Sent: den 28 januari 2010 11:05
To: [hidden email]
Subject: Re: how to measure dimensions of middle ear structure

The "Plugins - Transform - Interactive Stack Rotation" will do it:

1. Open a stack
2. Run the plugin
3. push 'F1' to see all mouse and key bindings to rotate the stack
4. when done rotating, make sure the window is active and push 'enter'
to convert it to a regular ImageJ stack.
5. Draw a line ROI and push 'm' to measure it.

It's in Fiji:  http://pacific.mpi-cbg.de

Albert
--
http://albert.rierol.net