overlay/merge images

[chen li]

Dear all,

I have two images in jpg format: one is taken by normal light/bright field and the other is taken by green light/green filter. I want to overlay the pictures together. When I check  ImageJ using color-->merge function, I can see 3 colors: Red, green, and blue. There is no normal field/bright field choice for me. I wonder if it is possible to merge/overlay two pictures, one of which is bright field picture and the other is green.


Thank you so much in advance,


Li


 


     

[Jacqueline Ross]

Dear Li,

If you have a recent version of ImageJ, then there is a channel
available for grey images, i.e. brightfield = grey.

Upgrade your version and try again. You should then see Red, Green, Blue
and Gray.

Kind regards,

Jacqui

Jacqueline Ross

Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND

Tel: [phone] Ext 87438
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
chen li
Sent: Friday, 21 August 2009 1:03 p.m.
To: [hidden email]
Subject: overlay/merge images

Dear all,

I have two images in jpg format: one is taken by normal light/bright
field and the other is taken by green light/green filter. I want to
overlay the pictures together. When I check  ImageJ using color-->merge
function, I can see 3 colors: Red, green, and blue. There is no normal
field/bright field choice for me. I wonder if it is possible to
merge/overlay two pictures, one of which is bright field picture and the
other is green.


Thank you so much in advance,


Li


 


     

[Joel Sheffield]

It might be that you have a very old version of ImageJ.  However, you should
be careful when merging color and greyscale images.  Take a look at the
powerpoint tutorial that I posted at
http://rsbweb.nih.gov/ij/docs/examples/IJ-M&M08.ppt.  There is a section on
color and merging images.
Joel


On Thu, Aug 20, 2009 at 9:02 PM, chen li <[hidden email]> wrote:

> Dear all,
>
> I have two images in jpg format: one is taken by normal light/bright field
> and the other is taken by green light/green filter. I want to overlay the
> pictures together. When I check  ImageJ using color-->merge function, I can
> see 3 colors: Red, green, and blue. There is no normal field/bright field
> choice for me. I wonder if it is possible to merge/overlay two pictures, one
> of which is bright field picture and the other is green.
>
>
> Thank you so much in advance,
>
>
> Li
>
>
>
>
>
>
>

--


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs